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|Title:||A method to isolate and purify human bone marrow stromal stem cells|
|Citation:||Mesenchymal Stem Cells: Methods and Protocols, 2008 / Prockop, D., Phinney, D., Bunnell, B. (ed./s), pp.45-57|
|Publisher Place:||United States|
|Stan Gronthos and Andrew C. W. Zannettino|
|Abstract:||The STRO-1 antibody can be used as a single reagent to isolate human bone marrow stromal stem cells (BMSSC), owing to its restricted specificity to a cell surface molecule expressed by clonogenic BMSSC, with little or no reactivity to hematopoietic stem/progenitor populations or mature stromal elements. The present protocol uses a combination of two different immunoselection methodologies in an attempt to generate highly purified preparations of BMSSC. This process involves the initial isolation of a minor subpopulation of bone marrow mononuclear cells (approx 10%) expressing the STRO-1 antigen, by means of magnetic activated cell sorting. Dual-color fluorescence activated cell sorting is then used as a secondary step to further purify the rare STRO-1bright expressing fraction that contains all of the colony-forming BMSSC, based on their co-expression of a secondary cell surface marker, CD106 (VCAM-1).|
|Keywords:||Bone marrow stromal stem cells; mesenchymal stem cells; magnetic activated cell sorting; fluorescence activated cell sorting|
|Description:||The original publication can be found at www.springerlink.com|
|Appears in Collections:||Medicine publications|
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