Please use this identifier to cite or link to this item:
Scopus Web of ScienceĀ® Altmetric
Type: Journal article
Title: Unusual Chromatin structural organization in the sperm head of a murid rodent from Southern Africa: the red veld rat, Aethomys chrysophilus type B
Author: Breed, W.
Citation: Reproduction, 1997; 111(2):221-228
Issue Date: 1997
ISSN: 0022-4251
Abstract: The structural organization of the chromatin of cauda epididymal spermatozoa of the red veld rat Aethomys chrysophilus type B was investigated by fluorescence microscopy after staining with DNA specific dyes and by transmission electron microscopy after incubation with Triton X100, dithiothreitol, and SDS. Staining with DNA dyes showed variation in intensity of fluorescence of the sperm chromatin, with an anterior spherical region staining far more intensely than the surrounding chromatin. Transmission electron microscopy of these spermatozoa indicated that this region was composed of cords and fibres. This chromatin region dispersed more readily than the surrounding chromatin when spermatozoa were incubated with the detergents, and it is suggested that, unlike the rest of the sperm chromatin, it may be a histone-rich region, with protamine(s) being either scarce or absent.
Keywords: Sperm Head
Fluorescent Dyes
Microscopy, Electron
Microscopy, Fluorescence
Microscopy, Phase-Contrast
Cell Culture Techniques
DOI: 10.1530/jrf.0.1110221
Appears in Collections:Anatomical Sciences publications
Aurora harvest
Environment Institute publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.