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Type: Journal article
Title: Regulation of epithelial apical junctions and barrier function by Gα13
Other Titles: Regulation of epithelial apical junctions and barrier function by G alpha13
Author: Donato, R.
Wood, S.
Saunders, I.
Gundsambuu, B.
Mak, K.
Abbott, C.
Powell, B.
Citation: BBA: Molecular Cell Research, 2009; 1793(7):1228-1235
Publisher: Elsevier Science BV
Issue Date: 2009
ISSN: 0167-4889
Statement of
Rino Donato, Stephen A. Wood, Ian Saunders, Batjargal Gundsambuu, Kai Yan Mak, Catherine A. Abbott and Barry C. Powell
Abstract: The epithelial tight junction forms a barrier to paracellular solute movement. In this study we show that the heterotrimeric G-protein Galpha13 regulates the epithelial tight junction barrier. We generated MDCKII kidney epithelial cell lines in which the expression of an active Galpha13 mutant (Galpha13Q226L) could be induced. We demonstrated that Galpha13Q226L expression increased paracellular permeability and caused the disruption and redistribution of proteins comprising the tight junction and the adherens junction away from sites of cell contact and the appearance of basal stress fibers. The effects on the junctional proteins and the actin cytoskeleton were abrogated by the Rho kinase inhibitor Y27632 but not by the Src kinase inhibitor PP2. The Galpha13 mediated increase in permeability was also Src kinase independent but was partly dependent on Rho kinase signalling. Our data establish a link between Galpha13, Rho kinase signaling and epithelial barrier function and not only demonstrate that Galpha13 regulates epithelial apical junction properties but that it does so via signaling pathways that are distinct from the closely related protein Galpha12.
Keywords: Gα13
Cell junction
Rho kinase
DOI: 10.1016/j.bbamcr.2009.04.008
Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

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