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Type: Journal article
Title: Evaluation of three automated chromogenic FVIII kits for the diagnosis of mild discrepant haemophilia A
Author: Rodgers, S.
Duncan, E.
Sobieraj-Teague, M.
Lloyd, J.
Citation: International Journal of Laboratory Hematology, 2009; 31(2):180-188
Publisher: Blackwell Publishing Ltd
Issue Date: 2009
ISSN: 1751-5521
Statement of
S. E. Rodgers, E. M. Duncan, M. Sobieraj-Teague, J. V. Lloyd
Abstract: In some mild haemophilia A patients (discrepant phenotype), coagulation FVIII levels by one-stage assay (FVIII-1st) are more than double those by classical two-stage coagulation assay (FVIII-2st), and may fall within the normal range. Our aim was to assess automated two-stage chromogenic FVIII assays (FVIII-chr) for diagnosis of mild discrepant haemophilia A. Three chromogenic FVIII kits (Biophen, Coamatic and Dade-Behring) were evaluated, using recommended and extended incubation times. Samples were tested from patients with discrepant haemophilia (n = 7) and equivalent mild haemophilia (agreement between FVIII-1st and FVIII-2st, n = 4). For equivalent haemophilia, FVIII-chr were consistent with FVIII-1st and FVIII-2st for all kits at all incubation times. For discrepant haemophilia, using recommended incubation times, mean FVIII-chr using Biophen reagents was 22 IU/dl (range 13-31), with Coamatic 26 (17-34) and with Dade-Behring 41 (33-47), compared with 36 (27-44) for FVIII-1st and 8 (6-9) for FVIII-2st. FVIII-chr decreased as incubation time was increased with Biophen and Coamatic, but decreased less with Dade-Behring. FVIII-chr using the Dade-Behring kit gave similar results to FVIII-1st and is not suitable for diagnosis of mild discrepant haemophilia A. FVIII-chr by Biophen and Coamatic kits is suitable for diagnosis of these patients, especially with an extended incubation time.
Keywords: Chromogenic FVIII
discrepant mild haemophilia
FVIII assay
two-stage FVIII
DOI: 10.1111/j.1751-553X.2007.01021.x
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