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https://hdl.handle.net/2440/55104
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dc.contributor.author | Shoubridge, C. | - |
dc.contributor.author | Read, L. | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | Endocrinology, 2003; 144(5):1887-1893 | - |
dc.identifier.issn | 0013-7227 | - |
dc.identifier.issn | 1945-7170 | - |
dc.identifier.uri | http://hdl.handle.net/2440/55104 | - |
dc.description.abstract | During early postnatal development, the intestine is highly responsive to LR3IGF-I administration but refractory to IGF-I, in contrast to the mature intestine. Given that LR3IGF-I is an IGF-I analog that binds poorly to IGF binding proteins, the response of the intestine is likely to reflect regulation of IGF-I bioactivity by IGF binding proteins. This study measures the delivery of exogenous IGF-I peptides to the intestine in preweaning (d-19) and adult rats to determine whether a correlation exists with the potency advantage of LR3IGF-I in the intestine during postnatal development. IGF-I or LR3IGF-I (2.6 µg/kg) was spiked with corresponding 125I-labeled peptide (10 x 106 cpm) and administered iv as a bolus (n = 5–6/group) with blood and tissue samples collected 5 and 10 min post injection. In both age groups, the levels of 125I-IGF-I retained in the blood at both 5 and 10 min were higher than the levels of 125I-LR3IGF-I, consistent with the slower clearance rate for the native peptide. In the gastrointestinal tract, the levels of 125I-LR3IGF-I per gram of tissue were 37–50% higher than 125I-IGF-I. Surprisingly, there was little difference in the relative delivery of LR3IGF-I to IGF-I to the intestine, across developmental age. Although bolus iv-injected LR3IGF-I was cleared more rapidly from the circulation than IGF-I and was subsequently delivered to the intestine in higher amounts than the native peptide, the ratio of LR3IGF-I to IGF-I in gut tissues was approximately 2:1 in both age groups. Hence, selective delivery to the gut is unlikely to explain the markedly higher potency of 125I-LR3IGF-I in stimulating growth of the preweaning vs. adult intestine. | - |
dc.description.statementofresponsibility | C. A. Shoubridge and L. C. Read | - |
dc.language.iso | en | - |
dc.publisher | Endocrine Soc | - |
dc.source.uri | http://dx.doi.org/10.1210/en.2002-220643 | - |
dc.subject | Intestinal Mucosa | - |
dc.subject | Animals | - |
dc.subject | Animals, Suckling | - |
dc.subject | Humans | - |
dc.subject | Rats | - |
dc.subject | Rats, Wistar | - |
dc.subject | Insulin-Like Growth Factor I | - |
dc.subject | Peptide Fragments | - |
dc.subject | Insulin-Like Growth Factor Binding Proteins | - |
dc.subject | Biological Availability | - |
dc.subject | Aging | - |
dc.subject | Female | - |
dc.title | Preferential intestinal delivery of long[arg(3)] Insulin-like growth factor (LR(3)IGF-I) over IGF-I in preweaning and adult rats | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1210/en.2002-220643 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Shoubridge, C. [0000-0002-0157-3084] | - |
Appears in Collections: | Aurora harvest 5 Paediatrics publications |
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