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Type: Journal article
Title: Anti-genotoxic effects of tea catechins against reactive oxygen species in human lymphoblastoid cells
Author: Sugisawa, A.
Kimura, M.
Fenech, M.
Umegaki, K.
Citation: Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 2004; 559(1-2):97-103
Publisher: Elsevier Science BV
Issue Date: 2004
ISSN: 1383-5718
Statement of
Ayako Sugisawa, Michiyo Kimura, Michael Fenech and Keizo Umegaki
Abstract: Using the cytokinesis-block micronucleus assay in WIL2-NS cells, we investigated the effects of six tea constituents, (−)-epigallocatechin-3-O-gallate (EGCg), (−)-epicatechin-3-O-gallate (ECg), (−)-epigallocatechin (EGC), (−)-epicatechin (EC), (+)-catechin (+C) and gallic acid (GA), on chromosomal damage in two ways; induction by each component on its own and prevention against treatment of reactive oxygen species (ROS). None of the tea constituents induced chromosomal damage at <10μM. On the other hand, EGCg, EGC, ECg, +C and GA prevented H₂O₂-induced chromosomal damage in a dose-dependent manner with a significant effect detected at 10 μM. Chromosomal damage induced by tert-butylhydroperoxide was apparently prevented by EGCg and ECg at 0.3 μM, but not by EGC and GA even at 10 μM, suggesting that the galloyl group linked to flavan-3-ol is needed for the observed protective effect. These results suggest that physiological concentration of tea constituents are not genotoxic but rather anti-genotoxic against ROS, although their preventive effects are slightly different depending on their chemical structure.
Keywords: Tea catechins
Chromosomal damage
Micronucleus assay
WIL2-NS cells
Reactive oxygen species
DOI: 10.1016/j.mrgentox.2004.01.002
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Pharmacology publications

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