Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/55192
Citations
Scopus Web of Science® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorDyer, Anthony R.en
dc.contributor.authorUpton, Zeeen
dc.contributor.authorStone, David Alan Josephen
dc.contributor.authorThomas, Philip Marken
dc.contributor.authorSoole, Kathleen Lydiaen
dc.contributor.authorHiggs, Naomien
dc.contributor.authorQuinn, K. J.en
dc.contributor.authorCarragher, John F.en
dc.date.issued2004en
dc.identifier.citationGeneral and Comparative Endocrinology, 2004; 135(3):268-275en
dc.identifier.issn0016-6480en
dc.identifier.urihttp://hdl.handle.net/2440/55192-
dc.description.abstractThis paper describes the development and validation of a commercially available radioimmunoassay (RIA) for the detection of fish insulin-like growth factor-I (IGF-I). The assay was developed using recombinant barramundi IGF-I as antigen and recombinant tuna IGF-I as radiolabelled tracer and standard. Assay sensitivity was 0.15 ng/ml, inter-assay variation was 16% (n=9) and intra-assay variation was 3% (n=10). Cross reactivity of less than 0.01% was found with salmon insulin, salmon IGF-II and barramundi IGF-II, less than 0.5% with human IGF-I and less than 1% with human IGF-II. Parallel dose-response inhibition curves were shown for barramundi (Lates calcarifer), coho salmon (Oncorhynchus kisutch), Southern Bluefin tuna (Thunnus maccoyii), tilapia (Oreochromis mossambicus), and seabream (Pagrus auratus) IGF-I. The assay was then used to measure stress related changes in different aquacultured fish species. Salt water acclimated Atlantic salmon smolts (Salmo salar) bathed for 2 h in fresh water showed significantly lower IGF-I concentrations than control smolts two days after the bath (53.1 compared to 32.1 ng/ml), with levels of IGF-I also lower in smolts exhibiting stunted growth (stunts). Capture and confinement of wild tuna in sea-cages resulted in a significant decrease in IGF-I levels (28 ng/ml) when compared to tuna captured and sampled immediately (48 ng/ml), but had recovered to starting levels after 3 weeks (43 ng/ml). Handling and isolation in silver perch (Bidyanus bidyanus) led to a gradual decline in IGF-I over a 12 h period (36–19 ng/ml) but showed signs of recovery by 24 h (24 ng/ml) and had recovered fully 72 h after treatment (40 ng/ml). A similar trial in black bream (Acanthopagrus butcherii) showed comparable results with IGF-I levels gradually decreasing (40–26 ng/ml) over 24 h, results that were mirrored by cortisol concentrations which increased during this time (1–26 ng/ml). In the studies presented here changes in IGF-I levels were not observed for at least 3 h after exposure to the stressor. We suggest this is due to the endocrine nature of IGF-I regulation and the clearance rate of IGF-I in vivo.en
dc.description.statementofresponsibilityAnthony R Dyer, Zee Upton, David Stone, Philip M Thomas, Kathleen L Soole, Naomi Higgs, Kirsty Quinn and John F Carragheren
dc.description.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/622837/description#descriptionen
dc.language.isoenen
dc.publisherElsevieren
dc.titleDevelopment and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levelsen
dc.typeJournal articleen
dc.contributor.schoolSchool of Earth and Environmental Sciences : Ecology and Evolutionary Biologyen
dc.identifier.doi10.1016/j.ygcen.2003.10.002en
Appears in Collections:Ecology, Evolution and Landscape Science publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.