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dc.contributor.authorIsola, D.en
dc.contributor.authorPardini, M.en
dc.contributor.authorVaraine, F.en
dc.contributor.authorNiemann, S.en
dc.contributor.authorRusch-Gerdes, S.en
dc.contributor.authorFattorini, L.en
dc.contributor.authorOrefici, G.en
dc.contributor.authorMeacci, F.en
dc.contributor.authorTrappetti, C.en
dc.contributor.authorOggioni, M.en
dc.contributor.authorOrru, G.en
dc.identifier.citationJournal of Microbiological Methods, 2005; 62(1):113-120en
dc.descriptionCopyright © 2005 Elsevier B.V. All rights reserved.en
dc.description.abstractEthambutol (EMB) is in use worldwide as a first-line anti-tuberculosis drug and substitutions in codon 306 of the embB gene are the most common mutations found in EMB resistant Mycobacterium tuberculosis (MTB) strains. Pyrosequencing is a real time sequencing method able to rapidly detect mutations in a large number of samples. Using this technique we analyzed, in parallel with conventional sequencing, a 24 bp region of the embB gene of 28 MTB clinical isolates. Pyrosequencing efficiently identified all embB306 mutations, detecting three different single-base substitutions leading to 2 amino acid changes (Met to Val or Ile). Mutated embB alleles were detected in 2 multidrug-resistant (MDR) EMB-susceptible strains. Overall, our results demonstrated that the Pyrosequencing method efficiently recognizes mutations in embB in a very short time and represents a valid molecular method to detect mutations in the MTB embB306 region.en
dc.description.statementofresponsibilityDaniela Isolaa, Manuela Pardinib, Francis Varainec, Stefan Niemanne, Sabine Rüsch-Gerdese, Lanfranco Fattorinib, Graziella Oreficib, Francesca Meaccid, Claudia Trappettid, Marco Rinaldo Oggionid, the LONG-DRUG study group, Germano Orrùen
dc.publisherElsevier Science BVen
dc.subjectEthambutol; embB; Mycobacterium tuberculosis; Pyrosequencing methoden
dc.titleA Pyrosequencing assay for rapid recognition of SNPs in Mycobacterium tuberculosis embB306 regionen
dc.typeJournal articleen
pubs.library.collectionMolecular and Biomedical Science publicationsen
dc.identifier.orcidTrappetti, C. [0000-0001-8272-0068]en
Appears in Collections:Molecular and Biomedical Science publications

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