Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/55843
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Type: Journal article
Title: Copper sensitivity of cueO mutants of Escherichia coli K-12 and the biochemical suppression of this phenotype
Author: Tree, J.
Kidd, S.
Jennings, M.
McEwan, A.
Citation: Biochemical and Biophysical Research Communications, 2005; 328(4):1205-1210
Publisher: Academic Press Inc
Issue Date: 2005
ISSN: 0006-291X
1090-2104
Statement of
Responsibility: 
Jai J. Tree, Stephen P. Kidd, Michael P. Jennings, Alastair G. McEwan
Abstract: The cueO gene of Escherichia coli encodes a multi-copper oxidase, which contributes to copper tolerance in this bacterium. It was observed that a cueO mutant was highly sensitive to killing by copper ions when cells were grown on defined minimal media. Copper sensitivity was correlated with accumulation of copper in the mutant strain. Growth of the cueO mutant in the presence of copper could be restored by addition of divalent zinc and manganese ions or ferrous iron but not by other first row transition metal ions or magnesium ions. Copper toxicity towards a cueO mutant could also be suppressed by addition of the superoxide quencher 1,2-dihydroxybenzene-3,5-disulfonic acid (tiron), suggesting that a primary cause of copper toxicity is the copper-catalyzed production of superoxide anions in the cytoplasm.
Keywords: Copper toxicity
Escherichia coli
Multi-copper oxidase
Description: Copyright © 2005 Elsevier Inc. All rights reserved.
DOI: 10.1016/j.bbrc.2005.01.084
Published version: http://dx.doi.org/10.1016/j.bbrc.2005.01.084
Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

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