Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/55920
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Comparative analysis of multidrug-resistant, non-multidrug-resistant, and archaic methicillin-resistant Staphylococcus aureus isolates from central Sydney, Australia
Author: Watson, J.
Givney, R.
Beard-Pegler, M.
Rose, B.
Merlino, J.
Vickery, A.
Gottlieb, T.
Bradbury, R.
Harbour, C.
Citation: Journal of Clinical Microbiology, 2003; 41(2):867-872
Publisher: Amer Soc Microbiology
Issue Date: 2003
ISSN: 0095-1137
1098-660X
Statement of
Responsibility: 
Jason Watson, Rod Givney, Mary Beard-Pegler, Barbara Rose, John Merlino, Alison Vickery, Tom Gottlieb, Ross Bradbury, and Colin Harbour
Abstract: In this study, the phenotypic and genotypic characteristics of 50 methicillin-resistant Staphylococcus aureus (MRSA) isolates (43 contemporary and 7 archaic strains from the mid-1960s) from four Sydney hospitals in the central Sydney area were compared. Phenotypic analysis based on antibiotic profiles and phage typing patterns categorized the MRSA isolates into three major groups: multidrug resistant (mMRSA), non-multidrug resistant (nmMRSA), and archaic. The nmMRSA isolates could be further subdivided into nmMRSA group 1, which was phage typeable and similar to the archaic group; nmMRSA group 2, which was non-phage typeable and only resistant to ciprofloxacin; and nmMRSA group 3, which was also nontypeable and generally resistant to other antibiotics. The characterization of all five phenotypic groups was then extended by genetic analysis. Restriction fragment length polymorphism (RFLP) analysis showed the 50 isolates could be sorted into 20 group-specific pulsotypes. mecI gene deletions and mutations at various percentages among the five MRSA groups were detected by sequencing. Several mec promoter mutations were also found. The overall findings indicated that nmMRSA strains may have independently acquired mec DNA and are more likely to be newly emergent strains than nmMRSA variants.
Keywords: Humans; Staphylococcus aureus; Anti-Bacterial Agents; Microbial Sensitivity Tests; Methicillin Resistance; Drug Resistance, Multiple; Genotype; Phenotype; Australia
Description: Copyright © 2003, American Society for Microbiology. All Rights Reserved.
RMID: 0020091805
DOI: 10.1128/JCM.41.2.867-872.2003
Appears in Collections:Public Health publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.