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Type: Journal article
Title: Functional expression cloning reveals proapoptotic role for protein phosphatase 4
Author: Mourtada-Maarabouni, M.
Kirkham, L.
Jenkins, B.
Rayner, J.
Gonda, T.
Starr, R.
Trayner, I.
Farzaneh, F.
Williams, G.
Citation: Cell Death and Differentiation, 2003; 10(9):1016-1024
Publisher: Nature Publishing Group
Issue Date: 2003
ISSN: 1350-9047
Statement of
M. Mourtada-Maarabouni, L. Kirkham, B. Jenkins, J. Rayner, T.J. Gonda, R. Starr, I. Trayner, F. Farzaneh and G.T. Williams
Abstract: Functional expression cloning strategies are highly suitable for the analysis of the molecular control of apoptosis. This approach has two critical advantages. Firstly, it eliminates prior assumptions about the properties of the proteins involved, and, secondly, it selectively targets proteins that are causally involved in apoptosis control and which affect the crucial cellular decision between survival and death. The application of this strategy to the isolation of cDNAs conferring resistance to dexamethasone and italic gamma-irradiation resulted in the isolation of a partial cDNA for the catalytic subunit of protein phosphatase 4 (PP4). Cells transfected with this partial cDNA in an expression vector downregulated PP4 and were resistant to both dexamethasone and UV radiation, as demonstrated by both membrane integrity and colony-forming assays. These observations suggest that PP4 plays an important proapoptotic role in T lymphocytes.
Keywords: PP4; dephosphorylation; phosphatase; apoptosis
Description: © 2003 Nature Publishing Group All rights reserved
RMID: 0020091832
DOI: 10.1038/sj.cdd.4401274
Appears in Collections:Medicine publications

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