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Type: Journal article
Title: Plasmid-borne bla(SHV) genes in Klebsiella pneumoniae are associated with strong promoters
Author: Turner, M.
Andersson, P.
Bell, J.
Turnidge, J.
Harris, T.
Giffard, P.
Citation: Journal of Antimicrobial Chemotherapy, 2009; 64(5):960-964
Publisher: Oxford Univ Press
Issue Date: 2009
ISSN: 0305-7453
Statement of
Mark S. Turner, Patiyan Andersson, Jan M. Bell, John D. Turnidge, Tegan Harris and Philip M. Giffard
Abstract: Background: Extended-spectrum β-lactamases (ESBLs) belonging to the SHV family remain a major cause of ESBL-positive phenotypes in Klebsiella pneumoniae. The blaSHV gene is a normal constituent of the K. pneumoniae chromosome. However, most ESBL-encoding blaSHV genes found in K. pneumoniae are plasmid borne. The objective was to determine the contribution of promoter variants to the expression of plasmid-borne blaSHV genes. Methods: K. pneumoniae clinical isolates were analysed for the presence of IS26 insertions characteristic of plasmid-borne blaSHV, and differences in their blaSHV promoter sequences and expression levels. A high resolution melting (HRM)-based method for rapid promoter analysis was developed. Results: An IS26 insertion characteristic of the plasmid-borne blaSHV-1/blaSHV-2/blaSHV-5 family was 100% linked to a promoter mutated in the –10 region, a mutation previously only found on the chromosome. The mutation was shown by real-time reverse transcriptase PCR to be associated with increased blaSHV expression. Conclusions: Plasmid-borne blaSHV is associated with strong promoters. It is likely that an SHV-dependent ESBL-positive phenotype requires both a strong promoter and a coding sequence mutation. An HRM assay can indicate blaSHV expression.
Keywords: K. pneumoniae
high resolution melting
DOI: 10.1093/jac/dkp338
Appears in Collections:Aurora harvest 5
Paediatrics publications

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