Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/56241
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Plasmid-borne bla(SHV) genes in Klebsiella pneumoniae are associated with strong promoters |
Author: | Turner, M. Andersson, P. Bell, J. Turnidge, J. Harris, T. Giffard, P. |
Citation: | Journal of Antimicrobial Chemotherapy, 2009; 64(5):960-964 |
Publisher: | Oxford Univ Press |
Issue Date: | 2009 |
ISSN: | 0305-7453 1460-2091 |
Statement of Responsibility: | Mark S. Turner, Patiyan Andersson, Jan M. Bell, John D. Turnidge, Tegan Harris and Philip M. Giffard |
Abstract: | Background: Extended-spectrum β-lactamases (ESBLs) belonging to the SHV family remain a major cause of ESBL-positive phenotypes in Klebsiella pneumoniae. The blaSHV gene is a normal constituent of the K. pneumoniae chromosome. However, most ESBL-encoding blaSHV genes found in K. pneumoniae are plasmid borne. The objective was to determine the contribution of promoter variants to the expression of plasmid-borne blaSHV genes. Methods: K. pneumoniae clinical isolates were analysed for the presence of IS26 insertions characteristic of plasmid-borne blaSHV, and differences in their blaSHV promoter sequences and expression levels. A high resolution melting (HRM)-based method for rapid promoter analysis was developed. Results: An IS26 insertion characteristic of the plasmid-borne blaSHV-1/blaSHV-2/blaSHV-5 family was 100% linked to a promoter mutated in the –10 region, a mutation previously only found on the chromosome. The mutation was shown by real-time reverse transcriptase PCR to be associated with increased blaSHV expression. Conclusions: Plasmid-borne blaSHV is associated with strong promoters. It is likely that an SHV-dependent ESBL-positive phenotype requires both a strong promoter and a coding sequence mutation. An HRM assay can indicate blaSHV expression. |
Keywords: | K. pneumoniae ESBLs SHV high resolution melting expression |
DOI: | 10.1093/jac/dkp338 |
Published version: | http://dx.doi.org/10.1093/jac/dkp338 |
Appears in Collections: | Aurora harvest 5 Paediatrics publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.