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https://hdl.handle.net/2440/56781
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Type: | Journal article |
Title: | Chaperone-targeting cytotoxin and endoplasmic reticulum stress-inducing drug synergize to kill cancer cells |
Author: | Backer, J. Krivoshein, A. Hamby, C. Pizzonia, J. Gilbert, K. Ray, Y. Brand, H. Paton, A. Paton, J. Backer, M. |
Citation: | Neoplasia, 2009; 11(11):1165-1173 |
Publisher: | Nature America Inc |
Issue Date: | 2009 |
ISSN: | 1522-8002 1476-5586 |
Statement of Responsibility: | Joseph M. Backer , Arcadius V. Krivoshein, Carl V. Hamby, John Pizzonia, Kenneth S. Gilbert, Yonaton S. Ray, Harrison Brand, Adrienne W. Paton, James Paton and Marina Backe |
Abstract: | Diverse physiological and therapeutic insults that increase the amount of unfolded or misfolded proteins in the ER induce the unfolded protein response, an evolutionarily conserved protective mechanism that manages ER stress. GRP78/BiP is an ER-resident protein that plays a central role in the ER stress response and is the only known substrate of the proteolytic A subunit (SubA) of a novel bacterial AB5 toxin. Here we report that an engineered fusion protein, EGF-SubA, combining EGF and SubA is highly toxic to growing and confluent EGFR-expressing cancer cells, and its cytotoxicity is mediated by a remarkably rapid cleavage of GRP78/BiP. Systemic delivery of EGF-SubA results in a significant inhibition of human breast and prostate tumor xenografts in mouse models. Furthermore, EGF-SubA dramatically increases the sensitivity of cancer cells to the ER stress-inducing drug thapsigargin, and vice-versa, demonstrating the first example of mechanism-based synergism in the action of a cytotoxin and an ER-targeting drug |
Keywords: | Endoplasmic Reticulum Animals Humans Mice Breast Neoplasms Prostatic Neoplasms Neoplasms, Experimental Epidermal Growth Factor Heat-Shock Proteins Recombinant Fusion Proteins Antineoplastic Agents Bacterial Toxins Microscopy, Fluorescence Blotting, Western Immunohistochemistry Xenograft Model Antitumor Assays Protein Folding Drug Synergism Female Male Stress, Physiological Endoplasmic Reticulum Chaperone BiP |
Description: | Copyright © 2009 Neoplasia Press, Inc. All rights reserved |
DOI: | 10.1593/neo.09878 |
Published version: | http://www.neoplasia.com/abstract.php?msid=2698 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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