Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/56800
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Characterization of bacterial and fungal biofilms in chronic rhinosinusitis
Author: Foreman, A.
Psaltis, A.
Tan, L.
Wormald, P.
Citation: American Journal of Rhinology & Allergy, 2009; 23(6):556-561
Publisher: Ocean Side Publications Inc
Issue Date: 2009
ISSN: 1945-8924
1945-8932
Statement of
Responsibility: 
Andrew Foreman, Alkis James Psaltis, Lor Wai Tan, Peter-John Wormald
Abstract: Background: Conclusive evidence exists that biofilms are present on the mucosa of chronic rhinosinusitis (CRS) patients. Less is known about the species constituting these biofilms. This study developed a fluorescence in situ hybridization (FISH) protocol for characterization of bacterial and fungal biofilms in CRS. Methods: Fifty CRS patients and 10 controls were recruited. Bacteria FISH probes for Staphylococcus aureus, Haemophilus influenzae, and Pseudomonas aeruginosa and a universal probe for fungi were applied to sinus mucosal specimens and then analyzed using confocal scanning laser microscopy. Results: Thirty-six of 50 CRS patients had biofilms present in contrast to 0/10 controls, suggesting a role for biofilms in the pathogenesis of this disease. S. aureus was the most common biofilm-forming organism. Eleven of 50 CRS patients had characteristic fungal biofilms present. Conclusion: This is the largest study of biofilms in CRS. It has validated mucosal tissue cryopreservation for delayed biofilm analysis. Fungal biofilms have been identified and the importance of S. aureus biofilms in the polymicrobial etiology of CRS is highlighted.
Keywords: Bacteria; biofilms; CRS; cryopreservation; FISH; fungi; pathogenesis; S. aureus
RMID: 0020094305
DOI: 10.2500/ajra.2009.23.3413
Description (link): http://www.ncbi.nlm.nih.gov/pubmed/19958600
Appears in Collections:Surgery publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.