Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/56836
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Type: Journal article
Title: Channel-like characteristics of the low affinity barley phosphate transporter Pht1;6 when expressed in Xenopus Oocytes1,[W],[OA]
Author: Preuss, C.
Huang, C.
Gilliham, M.
Tyerman, S.
Citation: Plant Physiology, 2010; 152(3):1431-1441
Publisher: Amer Soc Plant Physiologists
Issue Date: 2010
ISSN: 0032-0889
1532-2548
Organisation: Australian Centre for Plant Functional Genomics (ACPFG)
Statement of
Responsibility: 
Christian P. Preuss, Chun Y. Huang, Matthew Gilliham, and Stephen D. Tyerman
Abstract: Remobilization of inorganic phosphate (Pi) within a plant is critical for sustaining growth and seed production under external Pi fluctuation. The barley (Hordeum vulgare) transporter HvPHT1;6 has been implicated in Pi remobilization. In this report, we expressed HvPHT1;6 in Xenopus laevis oocytes, allowing detailed characterization of voltage-dependent fluxes and currents induced by HvPHT1;6. HvPHT1;6 increased efflux of Pi near oocyte resting membrane potentials, dependent on external Pi concentration. Time-dependent inward currents were observed when membrane potentials were more negative than –160 mV, which was consistent with nH+:HPO42– (n > 2) cotransport, based on simultaneous radiotracer and oocyte voltage clamping, dependent upon Pi concentration gradient and pH. Time- and voltage-dependent inward currents through HvPHT1;6 were also observed for SO42–and to a lesser degree for NO3–Cl–but not for malate. Inward and outward currents showed linear dependence on the concentration of external HPO42–similar to low-affinity Pi transport in plant studies. The electrophysiological properties of HvPHT1;6, which locates to the plasma membrane when expressed in onion (Allium cepa) epidermal cells, are consistent with its suggested role in the remobilization of Pi in barley plants.
Keywords: Oocytes; Animals; Xenopus; Hordeum; Phosphates; Phosphate Transport Proteins; Plant Proteins; DNA, Plant; Cloning, Molecular; Membrane Potentials
Rights: © 2010 American Society of Plant Biologists
RMID: 0020095990
DOI: 10.1104/pp.109.152009
Appears in Collections:Agriculture, Food and Wine publications

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