Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Channel-like characteristics of the low affinity barley phosphate transporter Pht1;6 when expressed in Xenopus Oocytes1,[W],[OA]|
|Citation:||Plant Physiology, 2010; 152(3):1431-1441|
|Publisher:||Amer Soc Plant Physiologists|
|Organisation:||Australian Centre for Plant Functional Genomics (ACPFG)|
|Christian P. Preuss, Chun Y. Huang, Matthew Gilliham, and Stephen D. Tyerman|
|Abstract:||Remobilization of inorganic phosphate (Pi) within a plant is critical for sustaining growth and seed production under external Pi fluctuation. The barley (Hordeum vulgare) transporter HvPHT1;6 has been implicated in Pi remobilization. In this report, we expressed HvPHT1;6 in Xenopus laevis oocytes, allowing detailed characterization of voltage-dependent fluxes and currents induced by HvPHT1;6. HvPHT1;6 increased efflux of Pi near oocyte resting membrane potentials, dependent on external Pi concentration. Time-dependent inward currents were observed when membrane potentials were more negative than –160 mV, which was consistent with nH+:HPO42– (n > 2) cotransport, based on simultaneous radiotracer and oocyte voltage clamping, dependent upon Pi concentration gradient and pH. Time- and voltage-dependent inward currents through HvPHT1;6 were also observed for SO42–and to a lesser degree for NO3–Cl–but not for malate. Inward and outward currents showed linear dependence on the concentration of external HPO42–similar to low-affinity Pi transport in plant studies. The electrophysiological properties of HvPHT1;6, which locates to the plasma membrane when expressed in onion (Allium cepa) epidermal cells, are consistent with its suggested role in the remobilization of Pi in barley plants.|
|Keywords:||Oocytes; Animals; Xenopus; Hordeum; Phosphates; Phosphate Transport Proteins; Plant Proteins; DNA, Plant; Cloning, Molecular; Membrane Potentials|
|Rights:||© 2010 American Society of Plant Biologists|
|Appears in Collections:||Agriculture, Food and Wine publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.