Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/56991
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Type: Journal article
Title: Regulation of the 5'-flanking region of the human CYP27B1 gene in osteoblast cells
Author: Turner, A.
Dwivedi, P.
Anderson, P.
May, B.
Morris, H.
Citation: Molecular and Cellular Endocrinology, 2009; 311(1-2):55-61
Publisher: Elsevier Sci Ireland Ltd
Issue Date: 2009
ISSN: 0303-7207
1872-8057
Statement of
Responsibility: 
A.G. Turner, P.P. Dwivedi, P.H. Anderson, B.K. May, H.A. Morris
Abstract: Synthesis of 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) is catalysed by the enzyme 25-hydroxyvitamin D(3)-1alpha-hydroxylase (CYP27B1). Regulation of CYP27B1 gene expression is poorly understood, particularly in non-renal tissues including bone where 1,25(OH)(2)D(3) is hypothesised to serve autocrine/paracrine roles. Transient transfection of ROS 17/2.8 osteoblast-like cells with reporter gene constructs containing deletions of the 5'-flanking region of the human CYP27B1 gene revealed a proximal promoter, enhancer region and strong upstream repressive region. Putative CCAAT and GC boxes, as well as Ets protein binding sites were shown to contribute to promoter and enhancer activities respectively in common with kidney and prostate cells. Inhibition of basal expression was largely attributed to a palindrome 5'-GTCTCAGAC-3' (-1015/-1007bp) that contains two putative canonical Smad binding elements. We conclude that repression of CYP27B1 gene expression may be a common event but the novel inhibitory elements we have identified may be unique to osteoblasts.
Keywords: Promoter; Smad; Repression; CYP27B1; Osteoblasts; Vitamin D
Description: Crown copyright © 2009 Published by Elsevier Ireland Ltd.
RMID: 0020092359
DOI: 10.1016/j.mce.2009.06.001
Appears in Collections:Molecular and Biomedical Science publications

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