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https://hdl.handle.net/2440/57211
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Type: | Journal article |
Title: | Cloning and characterization of an intracellular esterase from the wine-associated lactic acid bacterium Oenococcus oeni |
Author: | Sumby, K. Matthews, A. Grbin, P. Jiranek, V. |
Citation: | Applied and Environmental Microbiology, 2009; 75(21):6729-6735 |
Publisher: | Amer Soc Microbiology |
Issue Date: | 2009 |
ISSN: | 0099-2240 1098-5336 |
Statement of Responsibility: | Krista M. Sumby, Angela H. Matthews, Paul R. Grbin, and Vladimir Jiranek |
Abstract: | We report the cloning and characterization of EstB28, the first esterase to be so characterized from the wine-associated lactic acid bacterium, Oenococcus oeni. The published sequence for O. oeni strain PSU-1 was used to identify putative esterase genes and design PCR primers in order to amplify the corresponding region from strain Ooeni28, an isolate intended for inoculation of wines. In this way a 912-bp open reading frame (ORF) encoding a putative esterase of 34.5 kDa was obtained. The amino acid sequence indicated that EstB28 is a member of family IV of lipolytic enzymes and contains the GDSAG motif common to other lactic acid bacteria. This ORF was cloned into Escherichia coli using an appropriate expression system, and the recombinant esterase was purified. Characterization of EstB28 revealed that the optimum temperature, pH, and ethanol concentration were 40°C, pH 5.0, and 28% (vol/vol), respectively. EstB28 also retained marked activity under conditions relevant to winemaking (10 to 20°C, pH 3.5, 14% [vol/vol] ethanol). Kinetic constants were determined for EstB28 with p-nitrophenyl (pNP)-linked substrates ranging in chain length from C₂to C₁₈. EstB28 exhibited greatest specificity for C₂to C₄pNP-linked substrates. |
Keywords: | Escherichia coli Esterases Bacterial Proteins Recombinant Proteins DNA, Bacterial Cloning, Molecular Polymerase Chain Reaction Enzyme Stability Temperature Gene Expression Amino Acid Sequence Amino Acid Motifs Base Sequence Substrate Specificity Kinetics Open Reading Frames Hydrogen-Ion Concentration Molecular Weight Molecular Sequence Data Oenococcus |
DOI: | 10.1128/AEM.01563-09 |
Published version: | http://dx.doi.org/10.1128/aem.01563-09 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 5 |
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