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Type: Journal article
Title: Cloning and characterization of an intracellular esterase from the wine-associated lactic acid bacterium Oenococcus oeni
Author: Sumby, K.
Matthews, A.
Grbin, P.
Jiranek, V.
Citation: Applied and Environmental Microbiology, 2009; 75(21):6729-6735
Publisher: Amer Soc Microbiology
Issue Date: 2009
ISSN: 0099-2240
Statement of
Krista M. Sumby, Angela H. Matthews, Paul R. Grbin, and Vladimir Jiranek
Abstract: We report the cloning and characterization of EstB28, the first esterase to be so characterized from the wine-associated lactic acid bacterium, Oenococcus oeni. The published sequence for O. oeni strain PSU-1 was used to identify putative esterase genes and design PCR primers in order to amplify the corresponding region from strain Ooeni28, an isolate intended for inoculation of wines. In this way a 912-bp open reading frame (ORF) encoding a putative esterase of 34.5 kDa was obtained. The amino acid sequence indicated that EstB28 is a member of family IV of lipolytic enzymes and contains the GDSAG motif common to other lactic acid bacteria. This ORF was cloned into Escherichia coli using an appropriate expression system, and the recombinant esterase was purified. Characterization of EstB28 revealed that the optimum temperature, pH, and ethanol concentration were 40°C, pH 5.0, and 28% (vol/vol), respectively. EstB28 also retained marked activity under conditions relevant to winemaking (10 to 20°C, pH 3.5, 14% [vol/vol] ethanol). Kinetic constants were determined for EstB28 with p-nitrophenyl (pNP)-linked substrates ranging in chain length from C₂to C₁₈. EstB28 exhibited greatest specificity for C₂to C₄pNP-linked substrates.
Keywords: Escherichia coli; Esterases; Bacterial Proteins; Recombinant Proteins; DNA, Bacterial; Cloning, Molecular; Polymerase Chain Reaction; Enzyme Stability; Temperature; Gene Expression; Amino Acid Sequence; Amino Acid Motifs; Base Sequence; Substrate Specificity; Kinetics; Open Reading Frames; Hydrogen-Ion Concentration; Molecular Weight; Molecular Sequence Data; Oenococcus
RMID: 0020093062
DOI: 10.1128/AEM.01563-09
Appears in Collections:Agriculture, Food and Wine publications

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