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Type: Journal article
Title: The COG complex, Rab6 and COPI define a novel golgi retrograde trafficking pathway that is exploited by SubAB toxin
Author: Smith, R.
Willett, R.
Kudlyk, T.
Pokrovskaya, I.
Paton, A.
Paton, J.
Lupashin, V.
Citation: Traffic, 2009; 10(10):1502-1517
Publisher: Munksgaard Int Publ Ltd
Issue Date: 2009
ISSN: 1398-9219
Statement of
Richard D. Smith, Rose Willett, Tetyana Kudlyk, Irina Pokrovskaya, Adrienne W. Paton, James C. Paton and Vladimir V. Lupashin
Abstract: Toxin trafficking studies provide valuable information about endogenous pathways of intracellular transport. Subtilase cytotoxin (SubAB) is transported in a retrograde manner through the endosome to the Golgi and then to the endoplasmic reticulum (ER), where it specifically cleaves the ER chaperone BiP/GRP78 (Binding immunoglobin protein/Glucose-Regulated Protein of 78 kDa). To identify the SubAB Golgi trafficking route, we have used siRNA-mediated silencing and immunofluorescence microscopy in HeLa and Vero cells. Knockdown (KD) of subunits of the conserved oligomeric Golgi (COG) complex significantly delays SubAB cytotoxicity and blocks SubAB trafficking to the cis Golgi. Depletion of Rab6 and ╬▓-COP proteins causes a similar delay in SubAB-mediated GRP78 cleavage and did not augment the trafficking block observed in COG KD cells, indicating that all three Golgi factors operate on the same 'fast' retrograde trafficking pathway. SubAB trafficking is completely blocked in cells deficient in the Golgi SNARE Syntaxin 5 and does not require the activity of endosomal sorting nexins SNX1 and SNX2. Surprisingly, depletion of Golgi tethers p115 and golgin-84 that regulates two previously described coat protein I (COPI) vesicle-mediated pathways did not interfere with SubAB trafficking, indicating that SubAB is exploiting a novel COG/Rab6/COPI-dependent retrograde trafficking pathway.
Keywords: coiled-coil tether
conserved oligomeric Golgi complex
retrograde traffic
SubAB toxin
DOI: 10.1111/j.1600-0854.2009.00965.x
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Molecular and Biomedical Science publications

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