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dc.contributor.authorHayakawa, K.en
dc.contributor.authorHiramatsu, N.en
dc.contributor.authorOkamura, M.en
dc.contributor.authorYamazaki, H.en
dc.contributor.authorNakajima, S.en
dc.contributor.authorYao, J.en
dc.contributor.authorPaton, A.en
dc.contributor.authorPaton, J.en
dc.contributor.authorKitamura, M.en
dc.identifier.citationJournal of Immunology, 2009; 182(2):1182-1191en
dc.descriptionCopyright © 2009 by The American Association of Immunologists, Inc.en
dc.description.abstractAcute endoplasmic reticulum (ER) stress causes induction of inflammatory molecules via activation of NF-B. However, we found that, under ER stress conditions, renal mesangial cells acquire anergy to proinflammatory stimuli. Priming of the cells with ER stress inducers (tunicamycin, thapsigargin, A23187, and AB5 subtilase cytotoxin) caused blunted induction of MCP-1 in response to TNF-, IL-1β, macrophage-derived factors, or bystander macrophages. The magnitude of suppression was closely correlated with the level of GRP78, an endogenous indicator of ER stress. The suppression of MCP-1 under ER stress conditions was reversible and observed in general regardless of cell types or triggers of ER stress. The decrease in the level of MCP-1 mRNA was ascribed to transcriptional suppression via unexpected inhibition of NF-B, but not to accelerated mRNA degradation. Subsequent experiments revealed that TNFR-associated factor 2, an essential component for TNF- signaling, was down-regulated by ER stress. We also found that, under ER stress conditions, expression of NF-B suppressor A20 was induced. Overexpression of A20 resulted in suppression of cytokine-triggered NF-B activation and knockdown of A20 by RNA interference significantly attenuated induction of anergy by ER stress. In contrast, other ER stress-inducible/-related molecules that may suppress NF-B (e.g., GRP78, NO, reactive oxygen species, and IB) were not involved in the inhibitory effects of ER stress. These results elucidated ER stress-dependent mechanisms by which nonimmune cells acquire anergy to inflammatory stimuli under pathological situations. This self-defense machinery may play a role in halting progression of acute inflammation and in its spontaneous subsidenceen
dc.description.statementofresponsibilityKunihiro Hayakawa, Nobuhiko Hiramatsu, Maro Okamura, Hiroaki Yamazaki, Shotaro Nakajima, Jian Yao, Adrienne W. Paton, James C. Paton and Masanori Kitamuraen
dc.publisherAmer Assoc Immunologistsen
dc.subjectKidney Glomerulus; Cell Line; Cell Line, Transformed; Cell Line, Tumor; Endoplasmic Reticulum; Macrophages; Animals; Mice; Rats; Thapsigargin; NF-kappa B; Inflammation Mediators; Chemokines; Cytokines; Coculture Techniques; Clonal Anergy; Gene Expression Regulation; Mesangial Cells; Chemokine CCL2; Stress, Physiologicalen
dc.titleAcquisition of Anergy to Proinflammatory Cytokines in Nonimmune Cells through Endoplasmic Reticulum Stress Response: A Mechanism for Subsidence of Inflammationen
dc.typeJournal articleen
dc.identifier.orcidPaton, J. [0000-0001-9807-5278]en
Appears in Collections:Molecular and Biomedical Science publications

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