Please use this identifier to cite or link to this item:
Scopus Web of ScienceĀ® Altmetric
Type: Journal article
Title: Developmental expression of dermatan sulfate proteoglycans in the elastic bovine nuchal ligament
Author: Reinboth, B.
Finnis, M.
Gibson, M.
Sandberg, L.
Cleary, E.
Citation: Matrix Biology, 2000; 19(2):149-162
Publisher: Elsevier Science BV
Issue Date: 2000
ISSN: 0945-053X
Abstract: The nuchal ligament of bovines is a useful system in which to study elastic fibre formation since it contains up to 83% elastin and undergoes a period of rapid elastinogenesis during the last trimester of fetal development and in the first four post-natal months. To identify proteoglycans (PGs) which may be involved in this process we initially investigated changes in the glycosaminoglycan (GAG) profiles during nuchal ligament development. In contrast to the collagenous Achilles tendon, nuchal ligament exhibited: (a) elevated hyaluronan (HA) levels in the peak period of elastin-associated microfibril (fibrillin) synthesis (130-200 days) which precedes elastinogenesis; and (b) markedly increased synthesis of a glucuronate-rich copolymeric form of dermatan sulfate (DS) in the period corresponding to elastin formation (200-270 days). Analysis of DSPGs isolated from 230-day nuchal ligament showed that this copolymer was predominantly associated with a glycoform of biglycan which was specifically elevated at this stage in development. This finding was consistent with Northern blot analysis which showed that steady-state biglycan mRNA levels increased significantly during the elastinogenic period. In contrast, the mRNA levels for decorin, the only other DSPG detected in this tissue, declined rapidly after 140 days of fetal development. In conclusion, the results suggest that HA may play a role in microfibril assembly and that a specific glycoform of biglycan may be associated with the elastinogenic phase of elastic fibre formation.
Keywords: Ligaments
Elastic Tissue
Chondroitin Sulfates
Dermatan Sulfate
Heparitin Sulfate
Hyaluronic Acid
RNA, Messenger
Gene Expression Regulation, Developmental
Chondroitin Sulfate Proteoglycans
DOI: 10.1016/S0945-053X(00)00060-3
Published version:
Appears in Collections:Aurora harvest
Pathology publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.