Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Intranasal flu vaccine protective against seasonal and H5N1 avian influenza infections
Author: Alsharifi, M.
Furuya, Y.
Bowden, T.
Lobigs, M.
Koskinen, A.
Regner, M.
Trinidad, L.
Boyle, D.
Mullbacher, A.
Citation: PLoS One, 2009; 4(4):1-6
Publisher: Public Library of Science
Issue Date: 2009
ISSN: 1932-6203
Statement of
Mohammed Alsharifi, Yoichi Furuya, Timothy R. Bowden, Mario Lobigs, Aulikki Koskinen, Matthias Regner, Lee Trinidad, David B. Boyle and Arno Müllbacher
Abstract: Background Influenza A (flu) virus causes significant morbidity and mortality worldwide, and current vaccines require annual updating to protect against the rapidly arising antigenic variations due to antigenic shift and drift. In fact, current subunit or split flu vaccines rely exclusively on antibody responses for protection and do not induce cytotoxic T (Tc) cell responses, which are broadly cross-reactive between virus strains. We have previously reported that γ-ray inactivated flu virus can induce cross-reactive Tc cell responses. Methodology/Principal Finding Here, we report that intranasal administration of purified γ-ray inactivated human influenza A virus preparations (γ-Flu) effectively induces heterotypic and cross-protective immunity. A single intranasal administration of γ-A/PR8[H1N1] protects mice against lethal H5N1 and other heterotypic infections. Conclusions/Significance Intranasal γ-Flu represents a unique approach for a cross-protective vaccine against both seasonal as well as possible future pandemic influenza A virus infections.
Keywords: T-Lymphocytes, Cytotoxic
Mice, Inbred BALB C
RNA, Viral
Vaccines, Inactivated
Influenza Vaccines
Administration, Intranasal
Cross Reactions
Gamma Rays
Influenza, Human
Influenza A Virus, H5N1 Subtype
Influenza A Virus, H1N1 Subtype
Rights: Copyright: © 2009 Alsharifi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0005336
Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

Files in This Item:
File Description SizeFormat 
hdl_58906.pdf265.46 kBPublisher's PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.