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dc.contributor.authorBailey, L.en
dc.contributor.authorWallace, J.en
dc.contributor.authorPolyak, S.en
dc.identifier.citationArchives of Biochemistry and Biophysics, 2010; 496(1):45-52en
dc.description.abstractHolocarboxylase synthetase (HCS) governs the cellular fate of the essential micronutrient biotin (Vitamin H or B7). HCS is responsible for attaching biotin onto the biotin-dependent enzymes that reside in the cytoplasm and mitochondria. Evidence for an alternative role, viz the regulation of gene expression, has also been reported. Recent immunohistochemical studies reported HCS is primarily nuclear, inconsistent with the location of HCS activity. Improved understanding of biotin biology demands greater knowledge about HCS. Here, we investigated the localisation of HCS and its isoforms. Three variants were observed that differ at the N-terminus. All HCS isoforms were predominantly non-nuclear, consistent with the distribution of biotin protein ligase activity. Unlike the longer constructs, the Met(58) isoform was also detected in the nucleus--a novel observation suggesting shuttling activity between nucleus and cytoplasm. We resolved that the previous controversies in the literature are due to specificity and detection limitations that arise when using partially purified antibodies.en
dc.description.statementofresponsibilityL.M. Bailey, J.C. Wallace and S.W. Polyaken
dc.publisherAcademic Press Incen
dc.rightsCrown Copyright 2010 Published by Elsevier Inc. All rights reserved.en
dc.subjectHolocarboxylase synthetise; Enzyme; Antibody; Cellular localisation; Isoformsen
dc.titleHolocarboxylase synthetase: Correlation of protein localisation with biological functionen
dc.typeJournal articleen
pubs.library.collectionMolecular and Biomedical Science publicationsen
dc.identifier.orcidPolyak, S. [0000-0002-8458-5194]en
Appears in Collections:Molecular and Biomedical Science publications

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