Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/59925
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: An essential function for the centrosomal protein NEDD1 in zebrafish development
Author: Manning, J.
Lewis, M.
Koblar, S.
Kumar, S.
Citation: Cell Death and Differentiation, 2010; 17(8):1302-1314
Publisher: Nature Publishing Group
Issue Date: 2010
ISSN: 1350-9047
1476-5403
Statement of
Responsibility: 
JA Manning, M Lewis, SA Koblar and S Kumar
Abstract: The centrosome is the primary microtubule organising centre of the cell. It is composed of many proteins, some of which make up the core of the centrosome, whereas others are used for specific functions. Although the cellular roles of many centrosomal proteins are well defined, much less is known about their functions and the role of the centrosome in development. In this study we investigated the function of NEDD1, a critical component of the centrosome essential for microtubule nucleation, in zebrafish (Danio rerio) development. The zebrafish homologue of NEDD1 (zNEDD1) was cloned and found to have a similar localisation and function to mammalian NEDD1. We show that zNEDD1 is essential for survival, as a high level of knockdown was embryonic lethal. Partial knockdown of zNEDD1 caused abnormalities including an increase in mitotic and apoptotic cells. Pronounced phenotypic defects were seen in the brain, with a lack of defined brain structures, incomplete neural tube formation and a disorganisation of neurons. In addition, we show that a reduction in zNEDD1 resulted in the loss of c-tubulin at the centrosome. Our data thus demonstrate that zNEDD1 is critical for the recruitment of c-tubulin to the centrosome, and is essential for the proper development of zebrafish.
Keywords: NEDD1
centrosome
zebrafish
development
Rights: © 2010 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.
DOI: 10.1038/cdd.2010.12
Grant ID: NHMRC
Appears in Collections:Aurora harvest
Medicine publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.