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Type: Journal article
Title: Mapping of genomic regions associated with net form of net blotch resistance in barley
Author: Raman, H.
Platz, G.
Chalmers, K.
Raman, R.
Read, B.
Barr, A.
Moody, D.
Citation: Australian Journal of Agricultural Research, 2003; 54(11-12):1359-1367
Publisher: C S I R O Publishing
Issue Date: 2003
ISSN: 0004-9409
Abstract: Quantitative trait loci (QTLs) associated with resistance to net blotch and their chromosomal locations were determined from analyses of doubled haploid progeny of Alexis/Sloop, Arapiles/Franklin, Sloop/Halcyon, and recombinant inbred lines of Sloop-sib/Alexis. Five QTLs on chromosomes 2H, 3H, and 4H were found to be associated with seedling resistance to the net form of net blotch. In Arapiles/Franklin and Alexis/Sloop populations, 4 significant QTLs explaining 9–17% of the variation in net blotch resistance were detected on 2H and 3H. A major locus, QRpts4L accounting for 64% of the variation in infection type, was detected on 4H in the Sloop/Halcyon population. In Sloop/Halcyon, 2 microsatellite markers, EBmac0906 and GMS089, and AFLP marker P13/M50-108, co-segregated and detected maximum variability for net blotch resistance as revealed by bootstrap analysis. EBmac0906 and Bmac0181 were validated in F2 progeny of an Ant29/Halcyon population and reliably predicted phenotypes of 93% of lines resistant and susceptible to net blotch. These markers may be used within breeding programs to select alleles favourable for net blotch resistance derived from Halcyon.
Keywords: seedling resistance; Pyrenophora teres f. teres; QTL; validation
RMID: 0020031108
DOI: 10.1071/ar03026
Appears in Collections:Agriculture, Food and Wine publications

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