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Type: Journal article
Title: A method for determination of fruit-derived ascorbic, tartaric, oxalic and malic acids, and its application to the study of ascorbic acid catabolism in grapevines
Author: Melino, V.
Soole, K.
Ford, C.
Citation: Australian Journal of Grape and Wine Research, 2009; 15(3):293-302
Publisher: Australian Society of Viticulture and Oenology
Issue Date: 2009
ISSN: 1322-7130
Statement of
V.J. Melino, K.L. Soole and C.M. Ford
Abstract: Background and Aims:  The majority of the acidity of a grapevine (Vitis vinifera L.) berry is a result of the accumulation of l-tartaric (TA) and l-malic acids (MA). TA is synthesised from l-ascorbic acid (Asc, vitamin C), the metabolism of which is poorly characterised in grapevines. In a distinct pathway, oxalic acid (OA) is also formed from Asc degradation. The aim of this study was to develop a single method whereby the distribution of Asc and its catabolites from fruit and vegetative sources could be determined. Methods and Results:  Effective recoveries of total Asc, TA, OA and MA were achieved with this extraction method, while chromatographic separation was accomplished with reversed-phase high-performance liquid chromatography (RP-HPLC). These results demonstrate that Asc and its catabolites TA and OA rapidly accumulate in immature berries, and that the Asc to dehydroascorbate ratio increases with berry maturity. Conclusions:  A method for the simultaneous analysis of Asc, TA, OA and MA in fruits is provided; moreover, we have demonstrated its use to study their distribution in fruits, rachis, leaves and roots. Significance of the Study:  This method enables accurate monitoring of the accumulation of Asc, permitting further research towards understanding acid metabolism during berry ripening.
Keywords: ascorbate; berry development; catabolism; malic acid; oxalic acid; tartaric acid
Rights: © 2009 Australian Society of Viticulture and Oenology Inc.
RMID: 0020092769
DOI: 10.1111/j.1755-0238.2009.00062.x
Appears in Collections:Agriculture, Food and Wine publications

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