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Type: Journal article
Title: NHS-A isoform of the NHS gene is a novel interactor of ZO-1
Author: Sharma, S.
Koh, K.
Collin, C.
Dave, A.
McMellon, A.
Sugiyama, Y.
McAvoy, J.
Voss, A.
Gecz, J.
Craig, J.
Citation: Experimental Cell Research, 2009; 315(14):2358-2372
Publisher: Academic Press Inc Elsevier Science
Issue Date: 2009
ISSN: 0014-4827
Statement of
Shiwani Sharma, Katrina SY Koh, Caitlin Collin, Alpana Dave, Amy McMellon, Yuki Sugiyama, John W. McAvoy, Anne K. Voss, Jozef Gécz and Jamie E. Craig
Abstract: Mutations in the NHS (Nance-Horan Syndrome) gene lead to severe congenital cataracts, dental defects and sometimes mental retardation. NHS encodes two protein isoforms, NHS-A and -1A that display cell-type dependent differential expression and localization. Here we demonstrate that of these two isoforms, the NHS-A isoform associates with the cell membrane in the presence of intercellular contacts and it immunoprecipitates with the tight junction protein ZO-1 in MDCK (Madin Darby Canine Kidney) epithelial cells and in neonatal rat lens. The NHS-1A isoform however is a cytoplasmic protein. Both Nhs isoforms are expressed during mouse development. Immunolabelling of developing mouse with the anti-NHS antibody that detects both isoforms revealed the protein in the developing head including the eye and brain. It was primarily expressed in epithelium including neural epithelium and certain vascular endothelium but only weakly expressed in mesenchymal cells. In the epithelium and vascular endothelium the protein associated with the cell membrane and co-localized with ZO-1, which indirectly indicates expression of the Nhs-A isoform in these structures. Membrane localization of the protein in the lens vesicle similarly supports Nhs-A expression. In conclusion, the NHS-A isoform of NHS is a novel interactor of ZO-1 and may have a role at tight junctions. This isoform is important in mammalian development especially of the organs in the head.
Keywords: Tight junctions
Vascular endothelium
Mental retardation
Membrane-associated protein
Rights: Copyright © 2009 Elsevier Inc. All rights reserved.
DOI: 10.1016/j.yexcr.2009.05.008
Appears in Collections:Aurora harvest 5
Obstetrics and Gynaecology publications

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