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|Title:||Characterization of cardiac remodeling in a large animal "one-kidney, one-clip" hypertensive model|
|Citation:||Blood Pressure, 2010; 19(2):119-125|
|Publisher:||Taylor & Francis As|
|Dennis H. Lau, Lorraine Mackenzie, Arumuga Rajendram, Peter J. Psaltis, Douglas R. Kelly, Peter Spyropoulos, Yuan Zhang, Santosh A. Olakkengil, Christine H. Russell, Anthony G. Brooks, Randall J. Faull, David A. Saint, Darren J. Kelly, M. Mohan Rao, Stephen G. Worthley & Prashanthan Sanders|
|Abstract:||Objective. The aim of this study is to characterize cardiac remodeling in a large animal model of hypertension. Methods. 23 sheep were subjected to unilateral nephrectomy followed by clamping of the remaining renal artery to 60% (“one kidney-one clip”, 1K1C) 3 weeks later. Blood pressure (BP) was monitored invasively over 73±28 days. Cardiac function was assessed with magnetic resonance imaging and compared with 12 size-matched controls. Detailed atrial histopathological analysis was performed. Results. In the 1K1C animals, BP rose from baseline to reach a plateau by 4 weeks (systolic BP: 107±12 to 169±27, diastolic BP: 71±10 to 118±29 mmHg, both p< 0.0001); cardiac hypertrophy was significant when compared with controls with increased left ventricular weight [left ventricular (LV)/body wt: 2.7±0.5 vs 2.1±0.2 g/kg, p=0.01] as well as bi-atrial enlargement (right atrial, RA: 22.9±4.9 vs 15.7±2.8g, p=0.003; left atrial, LA: 35.5±6.7 vs 20.9±4.1g, p=0.0003); cardiac magnetic imaging demonstrated significantly increased LA volumes (end-diastolic volume: 42.9±6.8 vs 28.7±6.3 ml, p< 0.0001) and reduced LA ejection fraction (24.1±3.6 vs 31.6±3.0%, p=0.001) while LV function was relatively preserved (42.3±4.7 vs 46.4±4.1%, p=0.1); degeneration and necrosis of atrial myocytes were evident with increased atrial lymphocytic infiltration and interstitial fibrosis. Conclusions. The ovine 1K1C model produces reliable and reproducible hypertension with demonstrable cardiac end-organ damage.|
|Keywords:||Animal models; cardiac remodeling; hypertension; magnetic resonance imaging|
|Rights:||© 2010 Informa UK Ltd.|
|Appears in Collections:||Medicine publications|
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