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Type: Journal article
Title: Diversity and clonotypic composition of influenza-specific CD8⁺ TCR repertoires remain unaltered in the absence of Aire
Other Titles: Diversity and clonotypic composition of influenza-specific CD8(+) TCR repertoires remain unaltered in the absence of Aire
Author: Kedzierska, K.
Valkenburg, S.
Guillonneau, C.
Hubert, F.
Cukalac, T.
Curtis, J.
Stambas, J.
Scott, H.
Kedzierski, L.
Venturi, V.
Davenport, M.
Citation: European Journal of Immunology, 2010; 40(3):849-858
Publisher: Wiley-V C H Verlag GMBH
Issue Date: 2010
ISSN: 0014-2980
Statement of
Katherine Kedzierska, Sophie A. Valkenburg, Carole Guillonneau, Francois-Xavier Hubert, Tania Cukalac, Joan M. Curtis, John Stambas, Hamish S. Scott, Lukasz Kedzierski, Vanessa Venturi, and Miles P. Davenport
Abstract: TCR repertoire diversity is important for the protective efficacy of CD8(+) T cells, limiting viral escape and cross-reactivity between unrelated epitopes. The exact mechanism for selection of restricted versus diverse TCR repertoires is far from clear, although one thought is that the epitopes resembling self-peptides might select a limited array of TCR due to the deletion of autoreactive TCR. The molecule Aire promotes the expression of tissue-specific Ag on thymic medullary epithelial cells and the deletion of autoreactive cells, and in the absence of Aire autoreactive cells persist. However, the contribution of Aire-dependent peptides to the selection of the Ag-specific TCR repertoire remains unknown. In this study, we dissect restricted (D(b)NP(366)%(+)CD8(+)) and diverse (D(b)PA(224)%(+)CD8(+), K(d)NP(147)%(+)CD8(+)) TCR repertoires responding to three influenza-derived peptides in Aire-deficient mice on both B6 and BALB/c backgrounds. Our study shows that the number, qualitative characteristics and TCR repertoires of all influenza-specific, D(b)NP(366)%(+)CD8(+), D(b)PA(224)%(+)CD8(+) and K(d)NP(147)%(+)CD8(+) T cells are not significantly altered in the absence of Aire. This provides the first demonstration that the selection of an Ag-specific T-cell repertoire is not significantly perturbed in the absence of Aire.
Keywords: CD81⁺ T cells
T-cell receptor repertoire
Viral infection
Rights: Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
DOI: 10.1002/eji.200939918
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