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|Title:||Diversity and clonotypic composition of influenza-specific CD8⁺ TCR repertoires remain unaltered in the absence of Aire|
|Other Titles:||Diversity and clonotypic composition of influenza-specific CD8(+) TCR repertoires remain unaltered in the absence of Aire|
|Citation:||European Journal of Immunology, 2010; 40(3):849-858|
|Publisher:||Wiley-V C H Verlag GMBH|
|Katherine Kedzierska, Sophie A. Valkenburg, Carole Guillonneau, Francois-Xavier Hubert, Tania Cukalac, Joan M. Curtis, John Stambas, Hamish S. Scott, Lukasz Kedzierski, Vanessa Venturi, and Miles P. Davenport|
|Abstract:||TCR repertoire diversity is important for the protective efficacy of CD8(+) T cells, limiting viral escape and cross-reactivity between unrelated epitopes. The exact mechanism for selection of restricted versus diverse TCR repertoires is far from clear, although one thought is that the epitopes resembling self-peptides might select a limited array of TCR due to the deletion of autoreactive TCR. The molecule Aire promotes the expression of tissue-specific Ag on thymic medullary epithelial cells and the deletion of autoreactive cells, and in the absence of Aire autoreactive cells persist. However, the contribution of Aire-dependent peptides to the selection of the Ag-specific TCR repertoire remains unknown. In this study, we dissect restricted (D(b)NP(366)%(+)CD8(+)) and diverse (D(b)PA(224)%(+)CD8(+), K(d)NP(147)%(+)CD8(+)) TCR repertoires responding to three influenza-derived peptides in Aire-deficient mice on both B6 and BALB/c backgrounds. Our study shows that the number, qualitative characteristics and TCR repertoires of all influenza-specific, D(b)NP(366)%(+)CD8(+), D(b)PA(224)%(+)CD8(+) and K(d)NP(147)%(+)CD8(+) T cells are not significantly altered in the absence of Aire. This provides the first demonstration that the selection of an Ag-specific T-cell repertoire is not significantly perturbed in the absence of Aire.|
|Keywords:||CD81⁺ T cells; Influenza; T-cell receptor repertoire; Viral infection|
|Rights:||Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim|
|Appears in Collections:||Medicine publications|
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