Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/61300
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Type: Journal article
Title: Proteomic characterization of mesenchymal stem cell-like populations derived from ovine periodontal ligament, dental pulp, and bone marrow: Analysis of differentially expressed proteins
Author: Mrozik, K.
Zilm, P.
Bagley, C.
Hack, S.
Hoffmann, P.
Gronthos, S.
Bartold, P.
Citation: Stem Cells and Development, 2010; 19(10):1485-1499
Publisher: Mary Ann Liebert Inc Publ
Issue Date: 2010
ISSN: 1547-3287
1557-8534
Statement of
Responsibility: 
Krzysztof M. Mrozik, Peter S. Zilm, Christopher J. Bagley, Sandra Hack, Peter Hoffmann, Stan Gronthos and P. Mark Bartold
Abstract: Postnatal mesenchymal stem/stromal-like cells (MSCs) including periodontal ligament stem cells (PDLSCs), dental pulp stem cells (DPSCs), and bone marrow stromal cells (BMSCs) are capable of self-renewal and differentiation into multiple mesenchymal cell lineages. Despite their similar expression of MSC-associated and osteoblastic markers, MSCs retain the capacity to generate structures resembling the microenvironments from which they are derived in vivo and represent a promising therapy for the regeneration of complex tissues in the clinical setting. With this in mind, systematic approaches are required to identify the differential protein expression patterns responsible for lineage commitment and mediating the formation of these complex structures. This is the first study to compare the differential proteomic expression profiles of ex vivo-expanded ovine PDLSCs, DPSCs, and BMSCs derived from an individual donor. The two-dimensional electrophoresis was performed and regulated proteins were identified by liquid chromatography--electrospray-ionization tandem mass spectrometry (MS and MS/MS), database searching, and de novo sequencing. In total, 58 proteins were differentially expressed between at least 2 MSC populations in both sheep, 12 of which were up-regulated in one MSC population relative to the other two. In addition, the regulation of selected proteins was also conserved between equivalent human MSC populations. We anticipate that differential protein expression profiling will provide a basis for elucidating the protein expression patterns and molecular cues that are crucial in specifying the characteristic growth and developmental capacity of dental and non-dental tissue-derived MSC populations. These expression patterns can serve as important tools for the regeneration of particular tissues in future stem cell-based tissue engineering studies using animal models.
Keywords: Bone Marrow Cells; Periodontal Ligament; Dental Pulp; Animals; Sheep; Humans; Proteins; Proteome; Reproducibility of Results; Gene Expression Profiling; Regeneration; Databases, Protein; Female; Mesenchymal Stromal Cells
Rights: Copyright 2010 Mary Ann Liebert, Inc.
RMID: 0020100922
DOI: 10.1089/scd.2009.0446
Published version: http://find.galegroup.com/gtx/limitExpandSearchResults.do
Appears in Collections:Molecular and Biomedical Science publications

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