Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/61300
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dc.contributor.authorMrozik, K.en
dc.contributor.authorZilm, P.en
dc.contributor.authorBagley, C.en
dc.contributor.authorHack, S.en
dc.contributor.authorHoffmann, P.en
dc.contributor.authorGronthos, S.en
dc.contributor.authorBartold, P.en
dc.date.issued2010en
dc.identifier.citationStem Cells and Development, 2010; 19(10):1485-1499en
dc.identifier.issn1547-3287en
dc.identifier.issn1557-8534en
dc.identifier.urihttp://hdl.handle.net/2440/61300-
dc.description.abstractPostnatal mesenchymal stem/stromal-like cells (MSCs) including periodontal ligament stem cells (PDLSCs), dental pulp stem cells (DPSCs), and bone marrow stromal cells (BMSCs) are capable of self-renewal and differentiation into multiple mesenchymal cell lineages. Despite their similar expression of MSC-associated and osteoblastic markers, MSCs retain the capacity to generate structures resembling the microenvironments from which they are derived in vivo and represent a promising therapy for the regeneration of complex tissues in the clinical setting. With this in mind, systematic approaches are required to identify the differential protein expression patterns responsible for lineage commitment and mediating the formation of these complex structures. This is the first study to compare the differential proteomic expression profiles of ex vivo-expanded ovine PDLSCs, DPSCs, and BMSCs derived from an individual donor. The two-dimensional electrophoresis was performed and regulated proteins were identified by liquid chromatography--electrospray-ionization tandem mass spectrometry (MS and MS/MS), database searching, and de novo sequencing. In total, 58 proteins were differentially expressed between at least 2 MSC populations in both sheep, 12 of which were up-regulated in one MSC population relative to the other two. In addition, the regulation of selected proteins was also conserved between equivalent human MSC populations. We anticipate that differential protein expression profiling will provide a basis for elucidating the protein expression patterns and molecular cues that are crucial in specifying the characteristic growth and developmental capacity of dental and non-dental tissue-derived MSC populations. These expression patterns can serve as important tools for the regeneration of particular tissues in future stem cell-based tissue engineering studies using animal models.en
dc.description.statementofresponsibilityKrzysztof M. Mrozik, Peter S. Zilm, Christopher J. Bagley, Sandra Hack, Peter Hoffmann, Stan Gronthos and P. Mark Bartolden
dc.language.isoenen
dc.publisherMary Ann Liebert Inc Publen
dc.rightsCopyright 2010 Mary Ann Liebert, Inc.en
dc.source.urihttp://find.galegroup.com/gtx/limitExpandSearchResults.doen
dc.subjectBone Marrow Cells; Periodontal Ligament; Dental Pulp; Animals; Sheep; Humans; Proteins; Proteome; Reproducibility of Results; Gene Expression Profiling; Regeneration; Databases, Protein; Female; Mesenchymal Stromal Cellsen
dc.titleProteomic characterization of mesenchymal stem cell-like populations derived from ovine periodontal ligament, dental pulp, and bone marrow: Analysis of differentially expressed proteinsen
dc.typeJournal articleen
dc.identifier.rmid0020100922en
dc.identifier.doi10.1089/scd.2009.0446en
dc.identifier.pubid33268-
pubs.library.collectionMolecular and Biomedical Science publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidMrozik, K. [0000-0002-4890-8208]en
dc.identifier.orcidZilm, P. [0000-0001-7554-9717]en
dc.identifier.orcidGronthos, S. [0000-0002-6225-3084]en
dc.identifier.orcidBartold, P. [0000-0002-5695-3877]en
Appears in Collections:Molecular and Biomedical Science publications

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