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Type: Journal article
Title: Chronic maternal hypertension affects placental gene expression and differentiation in rabbits
Author: McArdle, A.
Maduwegedera, D.
Moritz, K.
Flower, R.
Denton, K.
Roberts, C.
Citation: Journal of Hypertension, 2010; 28(5):959-968
Publisher: Lippincott Williams & Wilkins
Issue Date: 2010
ISSN: 0263-6352
1473-5598
Statement of
Responsibility: 
Adelle M. McArdle, Devaki Maduwegedera, Karen Moritz, Rebecca L. Flower, Kate M. Denton and Claire T. Roberts
Abstract: BACKGROUND: Previously, we have shown that adult offspring from hypertensive rabbits develop hypertension. METHOD: We aimed to determine the effects of mild (+15 mmHg) and moderate (+25 mmHg) increases in maternal blood pressure and plasma renin activity on placental differentiation and expression of components of the renin-angiotensin system and 11[beta]-hydroxysteroid dehydrogenase type 2 mRNA in rabbits. Placentas were collected from normotensive (sham), mild (2-kidney-1-cellophane wrapped; 2K-1W) and moderate (2-kidney-2-cellophane wrapped; 2K-2W) hypertensive groups at gestational age of 14, 21 and 28 days. Placental gene expression was quantified by reverse transcriptase-PCR, and morphometry was assessed by videoimage analyses of placental sections. RESULTS: Fetal weight was similar between groups across gestation. In the 2K-1W group at gestational age day 14, fetal-to-placental weight ratio was increased (approximately 34%) as were volumes of fetal capillaries ([up arrow]56%) and maternal blood space at gestational age day 21 ([up arrow]55%) compared with sham (all P < 0.05). In the 2K-2W group, fetal-to-placental weight ratio was increased at gestational age day 21 (approximately 25%; P < 0.01) with an accompanying reduction in placental weight, and at gestational age day 28, volume density of fetal capillaries was increased (approximately 22%; P < 0.05). Placental renin mRNA was lower in both the 2K-1W (approximately 88%) and 2K-2W (approximately 98%) groups at gestational age day 28 (all P < 0.01). Placental 11[beta]-hydroxysteroid dehydrogenase type 2 mRNA was lower in the 2K-1W (approximately 36%) and 2K-2W (approximately 31%) groups at gestational age day 14 and greater (approximately 36%) in the 2K-2W group at gestational age day 21 (all P < 0.01). Associations between placental AT1R and AT2R mRNA and placental differentiation were disturbed by hypertension. CONCLUSION: Mild and moderate maternal hypertension differentially alters placental structure and gene expression that may affect placental functional capacity and contribute to programming of hypertension in offspring.
Keywords: chronic hypertension; placenta; pregnancy; programming; rabbit; renin-angiotensin system
Rights: © 2010 Lippincott Williams & Wilkins, Inc.
RMID: 0020096771
DOI: 10.1097/HJH.0b013e3283369f1e
Appears in Collections:Obstetrics and Gynaecology publications

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