Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Growth differentiation factor 9 signaling requires ERK1/2 activity in mouse granulosa and cumulus cells
Author: Sasseville, M.
Ritter, L.
Nguyen, T.
Liu, F.
Mottershead, D.
Russell, D.
Gilchrist, R.
Citation: Journal of Cell Science, 2010; 123(18):3166-3176
Publisher: Company of Biologists Ltd
Issue Date: 2010
ISSN: 0021-9533
Statement of
Maxime Sasseville, Lesley J. Ritter, Thao M. Nguyen, Fang Liu, David G. Mottershead, Darryl L. Russell and Robert B. Gilchrist
Abstract: Ovarian folliculogenesis is driven by the combined action of endocrine cues and paracrine factors. The oocyte secretes powerful mitogens, such as growth differentiation factor 9 (GDF9), that regulate granulosa cell proliferation, metabolism, steroidogenesis and differentiation. This study investigated the role of the epidermal growth factor receptor (EGFR)–extracellular signal-regulated kinase 1 and 2 (ERK1/2; also known as MAPK3/1) signaling pathway on GDF9 action on granulosa cells. Results show that mitogenic action of the oocyte is prevented by pharmacological inhibition of the EGFR–ERK1/2 pathway. Importantly, EGFR–ERK1/2 activity as well as rous sarcoma oncogene family kinases (SFK) are required for signaling through SMADs, mediating GDF9, activin A and TGFβ1 mitogenic action in granulosa cells. GDF9 could not activate ERK1/2 or affect EGF-stimulated ERK1/2 in granulosa cells. However, induction of the SMAD3-specific CAGA reporter by GDF9 in granulosa cells required active EGFR, SFKs and ERK1/2 as did GDF9-responsive gene expression. Finally, the EGFR–SFKs–ERK1/2 pathway was shown to be required for the maintenance of phosphorylation of the SMAD3 linker region. Together our results suggest that receptivity of granulosa cells to oocyte-secreted factors, including GDF9, is regulated by the level of activation of the EGFR and resulting ERK1/2 activity, through the requisite permissive phosphorylation of SMAD3 in the linker region. Our results indicate that oocyte-secreted TGFβ-like ligands and EGFR–ERK1/2 signaling are cooperatively required for the unique granulosa cell response to the signal from oocytes mediating granulosa cell survival and proliferation and hence the promotion of follicle growth and ovulation.
Keywords: Growth differentiation factor 9
Granulosa cells
Extracellular signal-regulated kinase 1/2
Cumulus cells
Rous sarcoma oncogene family kinases
Rights: © 2010. Published by The Company of Biologists
DOI: 10.1242/jcs.063834
Published version:
Appears in Collections:Aurora harvest
Obstetrics and Gynaecology publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.