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Type: Journal article
Title: Cytokine response of electrolytic ablation in an ex vivo perfused liver model
Author: Gravante, G.
Ong, S.
Metcalfe, M.
Sorge, R.
Overton, J.
Lloyd, D.
Maddern, G.
Dennison, A.
Citation: Australian and New Zealand Journal of Surgery, 2010; 80(7-8):537-541
Publisher: Blackwell Science Asia
Issue Date: 2010
ISSN: 1445-1433
Statement of
Gianpiero Gravante, Seok Ling Ong, Matthew S. Metcalfe, Roberto Sorge, John Overton, David M. Lloyd, Guy J. Maddern and Ashley R. Dennison
Abstract: <h4>Background</h4>The inflammatory response following hepatic ablation depends on different factors including the method used, the duration and intensity of the treatment and the presence or absence of ischemia. Debate continues about the use of different modalities and whether some aspects of the response may be advantageous by releasing immunological active substances. Little data have been published concerning the cytokine response elicited by hepatic electrolytic ablation (EA). Study of an ex vivo liver model could allow for the evaluation of this response without the influence of confounding systemic factors.<h4>Methods</h4>Livers explanted from 11 pigs were perfused extracorporeally with normothermic autologous blood. Four of them underwent EA after 1 h of reperfusion. Serum samples were obtained up to 6 h after the reperfusion and assayed for IL-1 beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IFN-gamma, TNF-alpha.<h4>Results</h4>Significant changes in the control group were observed for IL-6 after the second hour and IL-8 after the first hour compared with baseline levels (P < 0.001). In the EA group, IL-6 and IL-12 were raised after the second hour and IL-8 and IL-10 after the first hour (P < 0.001). The comparison between groups showed significant differences for IL-2, IL-4 (decreased in the EA group compared with controls), IL-10 and TNF-alpha (EA group increased compared with controls; P < 0.001).<h4>Conclusions</h4>The ex vivo perfused liver model demonstrated changes in levels of IL-2, IL-4, IL-10 and TNF-alpha following hepatic EA.
Keywords: ex vivo; liver; cytokine; inflammation; electrolytic ablation.
Rights: © 2010 The Authors. Journal compilation © 2010 Royal Australasian College of Surgeons
RMID: 0020100670
DOI: 10.1111/j.1445-2197.2010.05380.x
Appears in Collections:Surgery publications

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