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Type: Journal article
Title: Regulation of neutrophil-mediated killing of Staphylococcus aureus and chemotaxis by c-jun NH2 terminal kinase
Author: Yeh, M.
Mukaro, V.
Hii, C.
Ferrante, A.
Citation: Journal of Leukocyte Biology, 2010; 87(5):925-932
Publisher: Federation of American Societies for Experimental Biology
Issue Date: 2010
ISSN: 1938-3673
Statement of
Mei-Chun Yeh, Violet Mukaro, Charles S. Hii and Antonio Ferrante
Abstract: The role of JNK in neutrophil chemotaxis and killing of microbial pathogens remains unclear. Using a recently described cell-permeable peptide inhibitor of the JNK pathway, based on the JBD of JIP-1, coupled to the protein transduction domain of HIV-TAT (TAT-JIP), in association with control peptides, we demonstrate that the JNK pathway plays a major role in regulating human neutrophil chemotaxis and killing of microbial pathogens. Serum-opsonized Staphylococcus aureus elicited JNK activation and c-jun phosphorylation. The activation of the JNK pathway and bactericidal activity were inhibited by the TAT-JIP peptide. The stimulation of oxygen radical generation by S. aureus was dependent on the JNK signaling pathway, as was the phagocytosis of serum-opsonized bacteria. Chemotaxis to activated serum complement but not random migration was inhibited by the TAT-JIP peptide. The findings demonstrate a major role for the JNK signaling pathway in neutrophil-mediated defense against microbial pathogens.
Keywords: Neutrophils
HL-60 Cells
Staphylococcus aureus
Staphylococcal Infections
JNK Mitogen-Activated Protein Kinases
Enzyme Inhibitors
Blotting, Western
Chemotaxis, Leukocyte
Rights: © Society for Leukocyte Biology
DOI: 10.1189/jlb.0609399
Published version:
Appears in Collections:Aurora harvest
Paediatrics publications

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