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https://hdl.handle.net/2440/63001
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Type: | Journal article |
Title: | Effect of inactivation method on the cross-protective immunity induced by whole 'killed' influenza A viruses and commercial vaccine preparations |
Author: | Furuya, Y. Regner, M. Lobigs, M. Koskinen, A. Mullbacher, A. Alsharifi, M. |
Citation: | Journal of General Virology, 2010; 91(6):1450-1460 |
Publisher: | Soc General Microbiology |
Issue Date: | 2010 |
ISSN: | 0022-1317 1465-2099 |
Statement of Responsibility: | Yoichi Furuya, Matthias Regner, Mario Lobigs, Aulikki Koskinen, Arno Müllbacher and Mohammed Alsharifi |
Abstract: | We have recently shown that intranasal (i.n.) administration of {gamma}-irradiated A/PR/8 [A/Puerto Rico/8/34 (H1N1)] protects mice against lethal avian influenza A/Vietnam/1203/2004 (H5N1) and other heterosubtypic influenza A infections. Here, we used {gamma}-irradiated, formalin- and UV-inactivated A/PC [A/Port Chalmers/1/73 (H3N2)] virus preparations and compared their ability to induce both homologous and heterosubtypic protective immunity. Our data show that, in contrast to i.n. vaccination with formalin- or UV-inactivated virus, or the present commercially available trivalent influenza vaccine, a single dose of {gamma}-ray-inactivated A/PC ({gamma}-A/PC) conferred significant protection in mice against both homologous and heterosubtypic virus challenges. A multiple immunization regime was required for formalin-inactivated virus preparations to induce protective immunity against a homotypic virus challenge, but did not induce influenza A strain cross-protective immunity. The highly immunogenic {gamma}-A/PC, but not formalin- or UV-inactivated A/PC, nor the currently available subvirion vaccine, elicited cytotoxic T-cell responses that are most likely responsible for the cross-protective and long-lasting immunity against highly lethal influenza A infections in mice. Finally, freeze-drying of {gamma}-A/PC did not affect the ability to induce cross-protective immunity. |
Keywords: | Lung T-Lymphocytes, Cytotoxic Animals Mice, Inbred BALB C Mice Orthomyxoviridae Infections Body Weight Formaldehyde Vaccines, Inactivated Influenza Vaccines Viral Load Virus Inactivation Survival Analysis Ultraviolet Rays Gamma Rays Female Influenza A Virus, H3N2 Subtype Cross Protection |
Rights: | © 2010 SGM |
DOI: | 10.1099/vir.0.018168-0 |
Published version: | http://dx.doi.org/10.1099/vir.0.018168-0 |
Appears in Collections: | Aurora harvest 5 Microbiology and Immunology publications |
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