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Type: Journal article
Title: Effect of inactivation method on the cross-protective immunity induced by whole 'killed' influenza A viruses and commercial vaccine preparations
Author: Furuya, Y.
Regner, M.
Lobigs, M.
Koskinen, A.
Mullbacher, A.
Alsharifi, M.
Citation: Journal of General Virology, 2010; 91(6):1450-1460
Publisher: Soc General Microbiology
Issue Date: 2010
ISSN: 0022-1317
Statement of
Yoichi Furuya, Matthias Regner, Mario Lobigs, Aulikki Koskinen, Arno Müllbacher and Mohammed Alsharifi
Abstract: We have recently shown that intranasal (i.n.) administration of {gamma}-irradiated A/PR/8 [A/Puerto Rico/8/34 (H1N1)] protects mice against lethal avian influenza A/Vietnam/1203/2004 (H5N1) and other heterosubtypic influenza A infections. Here, we used {gamma}-irradiated, formalin- and UV-inactivated A/PC [A/Port Chalmers/1/73 (H3N2)] virus preparations and compared their ability to induce both homologous and heterosubtypic protective immunity. Our data show that, in contrast to i.n. vaccination with formalin- or UV-inactivated virus, or the present commercially available trivalent influenza vaccine, a single dose of {gamma}-ray-inactivated A/PC ({gamma}-A/PC) conferred significant protection in mice against both homologous and heterosubtypic virus challenges. A multiple immunization regime was required for formalin-inactivated virus preparations to induce protective immunity against a homotypic virus challenge, but did not induce influenza A strain cross-protective immunity. The highly immunogenic {gamma}-A/PC, but not formalin- or UV-inactivated A/PC, nor the currently available subvirion vaccine, elicited cytotoxic T-cell responses that are most likely responsible for the cross-protective and long-lasting immunity against highly lethal influenza A infections in mice. Finally, freeze-drying of {gamma}-A/PC did not affect the ability to induce cross-protective immunity.
Keywords: Lung; T-Lymphocytes, Cytotoxic; Animals; Mice, Inbred BALB C; Mice; Orthomyxoviridae Infections; Body Weight; Formaldehyde; Vaccines, Inactivated; Influenza Vaccines; Viral Load; Virus Inactivation; Survival Analysis; Ultraviolet Rays; Gamma Rays; Female; Influenza A Virus, H3N2 Subtype; Cross Protection
Rights: © 2010 SGM
RMID: 0020103180
DOI: 10.1099/vir.0.018168-0
Appears in Collections:Microbiology and Immunology publications

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