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Type: Journal article
Title: A SOS3 homologue maps to HvNax4, a barley locus controlling an environmentally sensitive Na⁺ exclusion trait
Other Titles: A SOS3 homologue maps to HvNax4, a barley locus controlling an environmentally sensitive Na+ exclusion trait
Author: Rivandi, A.
Miyazaki, J.
Hrmova, M.
Pallotta, M.
Tester, M.
Collins, N.
Citation: Journal of Experimental Botany, 2011; 62(3):1201-1216
Publisher: Oxford Univ Press
Issue Date: 2011
ISSN: 0022-0957
Statement of
J. Rivandi, J. Miyazaki, M. Hrmova, M. Pallotta, M. Tester and N. C. Collins
Abstract: Genes that enable crops to limit Na+ accumulation in shoot tissues represent potential sources of salinity tolerance for breeding. In barley, the HvNax4 locus lowered shoot Na+ content by between 12% and 59% (g−1 DW), or not at all, depending on the growth conditions in hydroponics and a range of soil types, indicating a strong influence of environment on expression. HvNax4 was fine-mapped on the long arm of barley chromosome 1H. Corresponding intervals of ∼200 kb, containing a total of 34 predicted genes, were defined in the sequenced rice and Brachypodium genomes. HvCBL4, a close barley homologue of the SOS3 salinity tolerance gene of Arabidopsis, co-segregated with HvNax4. No difference in HvCBL4 mRNA expression was detected between the mapping parents. However, genomic and cDNA sequences of the HvCBL4 alleles were obtained, revealing a single Ala111Thr amino acid substitution difference in the encoded proteins. The known crystal structure of SOS3 was used as a template to obtain molecular models of the barley proteins, resulting in structures very similar to that of SOS3. The position in SOS3 corresponding to the barley substitution does not participate directly in Ca2+ binding, post-translational modifications or interaction with the SOS2 signalling partner. However, Thr111 but not Ala111 forms a predicted hydrogen bond with a neighbouring α-helix, which has potential implications for the overall structure and function of the barley protein. HvCBL4 therefore represents a candidate for HvNax4 that warrants further investigation.
Keywords: Hordeum; Sodium; Plant Proteins; Arabidopsis Proteins; Chromosome Mapping; Sequence Alignment; Amino Acid Sequence; Molecular Conformation; Biological Transport; Quantitative Trait Loci; Molecular Sequence Data
Rights: © 2010 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( 2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0020101145
DOI: 10.1093/jxb/erq346
Appears in Collections:Agriculture, Food and Wine publications

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