Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/63285
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Type: Journal article
Title: Opposing effects of omega-3 and omega-6 long chain polyunsaturated fatty acids on the expression of lipogenic genes in omental and retroperitoneal adipose depots in the rat
Author: Muhlhausler, B.
Cook-Johnson, R.
James, M.
Miljkovic, D.
Duthoit, E.
Gibson, R.
Citation: Journal of Nutrition and Metabolism, 2010; 2010:1-9
Publisher: Hindawi Publishing Corporation
Issue Date: 2010
ISSN: 2090-0724
2090-0732
Statement of
Responsibility: 
B.S. Muhlhausler, R. Cook-Johnson, M. James, D. Miljkovic, E. Duthoit and R. Gibson
Abstract: This study aimed to determine the effect of varying dietary intake of the major n-3 PUFA in human diets, α-linolenic acid (ALA; 18 : 3n-3), on expression of lipogenic genes in adipose tissue. Rats were fed diets containing from 0.095%en to 6.3%en ALA and a constant n-6 PUFA level for 3 weeks. Samples from distinct adipose depots (omental and retroperitoneal) were collected and mRNA expression of the pro-lipogenic transcription factors Sterol-Retinoid-Element-Binding-Protein1c (SREBP1c) and Peroxisome Proliferator Activated Receptor-γ (PPARγ), lipogenic enzymes Sterol-coenzyme Desaturase1 (SCD-1), Fatty Acid Synthase (FAS), lipoprotein lipase (LPL) and glycerol-3-phosphate dehydrogenase (G3PDH) and adipokines leptin and adiponectin determined by qRT-PCR. Increasing dietary ALA content resulted in altered expression of SREBP1c, FAS and G3PDH mRNA in both adipose depots. SREBP1c mRNA expression was related directly to n-6 PUFA concentrations (omental, r2=.71; P<.001; Retroperitoneal, r2=.20; P<.002), and inversely to n-3 PUFA concentrations (omental, r2=.59; P<.001; Retroperitoneal, r2=.19; P<.005) independent of diet. The relationship between total n-6 PUFA and SREBP1c mRNA expression persisted when the effects of n-3 PUFA were controlled for. Altering red blood cell concentrations of n-3 PUFA is thus associated with altered expression of lipogenic genes in a depot-specific manner and this effect is modulated by prevailing n-6 PUFA concentrations.
Rights: Copyright © 2010 B. S. Muhlhausler et al.
RMID: 0020104044
DOI: 10.1155/2010/927836
Appears in Collections:Agriculture, Food and Wine publications

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