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dc.contributor.authorArthur, A.en
dc.contributor.authorZannettino, A.en
dc.contributor.authorPanagopoulos, R.en
dc.contributor.authorKoblar, S.en
dc.contributor.authorSims, N.en
dc.contributor.authorStylianou, C.en
dc.contributor.authorMatsuo, K.en
dc.contributor.authorGronthos, S.en
dc.identifier.citationBone, 2011; 48(3):533-542en
dc.description.abstractBone marrow derived mesenchymal stem/stromal cells (MSC) contribute to skeletal tissue formation and the regulation of haematopoiesis. The Eph/ephrin family of receptor tyrosine kinases is potentially important in the maintenance of the stem cell niche within neural, intestinal and dental tissues and has recently been shown to play a role in regulating bone homeostasis. However, the contribution of EphB/ephrin-B molecules in human MSC function remains to be determined. In the present study, EphB and ephrin-B molecules were expressed by ex vivo expanded human MSC populations and within human bone marrow trephine samples. To elucidate the contribution of EphB/ephrin-B molecules in MSC recruitment, we performed functional spreading and migration assays and showed that reverse ephrin-B signalling inhibited MSC attachment and spreading by activating Src-, PI3Kinase- and JNK-dependent signalling pathways. In contrast, forward EphB2 signalling promoted MSC migration by activating the Src kinase- and Abl-dependent signalling pathways. Furthermore, activation of ephrin-B1 and/or ephrin-B2 molecules expressed by MSC was found to increase osteogenic differentiation, while ephrin-B1 activation promoted chondrogenic differentiation. These observations suggest that EphB/ephrin-B interactions may mediate the recruitment, migration and differentiation of MSC during bone repair.en
dc.description.statementofresponsibilityAgnieszka Arthur, Andrew Zannettino, Romana Panagopoulos, Simon A. Koblar, Natalie A. Sims, Con Stylianou, Koichi Matsuo, Stan Gronthosen
dc.publisherElsevier Science Incen
dc.rightsCopyright © 2010 Elsevier Inc. All rights reserved.en
dc.subjectStem cell; Bone; Cartilage; Eph; Ephrin; Mesenchymal stem cellen
dc.titleEphB/ephrin-B interactions mediate human MSC attachment, migration and osteochondral differentiationen
dc.typeJournal articleen
pubs.library.collectionMedicine publicationsen
dc.identifier.orcidArthur, A. [0000-0002-0539-8797]en
dc.identifier.orcidZannettino, A. [0000-0002-6646-6167]en
dc.identifier.orcidKoblar, S. [0000-0002-8667-203X]en
dc.identifier.orcidGronthos, S. [0000-0002-6225-3084]en
Appears in Collections:Medicine publications

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