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Type: Journal article
Title: Growth characteristics and enzyme activity in Batrachochytrium dendrobatidis isolates
Author: Symonds, E.
Trott, D.
Bird, P.
Mills, P.
Citation: Mycopathologia, 2008; 166(3):143-147
Publisher: Kluwer Academic Publ
Issue Date: 2008
ISSN: 0301-486X
Statement of
E. Pearl Symonds, Darren J. Trott, Philip S. Bird, Paul Mills
Abstract: Batrachochytrium dendrobatidis is a member of the phylum Chytridiomycota and the causative organism chytridiomycosis, a disease of amphibians associated with global population declines and mass mortality events. The organism targets keratin-forming epithelium in adult and larval amphibians, which suggests that keratinolytic activity may be required to infect amphibian hosts. To investigate this hypothesis, we tested 10 isolates of B. dendrobatidis for their ability to grow on a range of keratin-supplemented agars and measured keratolytic enzyme activity using a commercially available kit (bioMerieux API ZYM). The most dense and fastest growth of isolates were recorded on tryptone agar, followed by growth on frog skin agar and the slowest growth recorded on feather meal and boiled snake skin agar. Growth patterns were distinctive for each nutrient source. All 10 isolates were strongly positive for a range of proteolytic enzymes which may be keratinolytic, including trypsin and chymotrypsin. These findings support the predilection of B. dendrobatidis for amphibian skin.
Keywords: Batrachochytrium dendrobatidis; Chytridiomycosis; Amphibian declines; Proteolytic enzyme
Rights: © Springer Science+Business Media B.V. 2008
RMID: 0020106754
DOI: 10.1007/s11046-008-9135-y
Appears in Collections:Agriculture, Food and Wine publications

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