Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/65723
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: TRPA1 contributes to specific mechanically activated currents and sensory neuron mechanical hypersensitivity
Author: Brierley, S.
Castro Kraftchenko, J.
Harrington, A.
Hughes, P.
Page, A.
Rychkov, G.
Blackshaw, L.
Citation: Journal of Physiology-London, 2011; 589(14):3575-3593
Publisher: Blackwell Publishing Ltd
Issue Date: 2011
ISSN: 0022-3751
1469-7793
Statement of
Responsibility: 
Stuart M Brierley, Joel Castro, Andrea M Harrington, Patrick A Hughes, Amanda J Page, Grigori Y Rychkov and L. Ashley Blackshaw
Abstract: The mechanosensory role of TRPA1 and its contribution to mechanical hypersensitivity in sensory neurons remains enigmatic. We elucidated this role by recording mechanically activated currents in conjunction with TRPA1 over-and under-expression and selective pharmacology. First, we established that TRPA1 transcript, protein and functional expression are more abundant in smaller-diameter neurons than larger-diameter neurons, allowing comparison of two different neuronal populations. Utilizing whole cell patch clamping, we applied calibrated displacements to neurites of DRG neurons in short-term culture and recorded mechanically activated currents termed Intermediately- (IAMC), Rapidly- (RAMC) or Slowly-Adapting (SAMC). TRPA1 deletion (-/-) significantly reduced maximum IAMC amplitude by 43% in small-diameter neurons compared with wild-type (+/+) neurons. All other mechanically activated currents in small- and large-diameter TRPA1-/- neurons were unaltered. 73% of TRPA1+/+ small-diameter neurons responding to the TRPA1 agonist AITC displayed IAMC to neurite displacement, which were significantly enhanced after AITC addition. The TRPA1 antagonist HC-030031 significantly decreased TRPA1+/+ IAMC amplitudes, but only in AITC responsive neurons. Using a transfection system we also showed TRPA1 over-expression in TRPA1+/+ small-diameter neurons increases IAMC amplitude, an effect reversed by HC-030031. Furthermore, TRPA1 introduction into TRPA1-/- small-diameter neurons restored IAMC amplitudes to TRPA1+/+ levels, which was subsequently reversed by HC-030031. In summary our data demonstrate TRPA1 makes a specific contribution to normal mechanosensation in a distinct subset of DRG neurons. Furthermore, they also provide new, direct evidence illustrating mechanisms by which sensitization or over-expression of TRPA1 enhances nociceptor mechanosensitivity. Overall, these findings suggest TRPA1 has the capacity to tune neuronal mechanosensitivity depending on its degree of activation or expression.
Keywords: Ganglia, Spinal; Cells, Cultured; Animals; Mice, Inbred C57BL; Mice, Knockout; Mice; Hyperalgesia; Acetanilides; Isothiocyanates; Purines; Patch-Clamp Techniques; Mechanotransduction, Cellular; Action Potentials; Transient Receptor Potential Channels; Sensory Receptor Cells
Rights: Copyright © 2011, The Physiological Society
RMID: 0020111266
DOI: 10.1113/jphysiol.2011.206789
Grant ID: http://purl.org/au-research/grants/nhmrc/508103
http://purl.org/au-research/grants/nhmrc/1008100
Description (link): http://jp.physoc.org/
Appears in Collections:Medicine publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.