Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/65840
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Type: Journal article
Title: Naturally occurring Sarcocystis neurona-like infection in a dog with myositis
Author: Vashisht, K.
Lichtensteigera, C.
Miller, L.
Gondim, L.
McAllister, M.
Citation: Veterinary Parasitology, 2005; 133(1):19-25
Publisher: Elsevier Science BV
Issue Date: 2005
ISSN: 0304-4017
1873-2550
Statement of
Responsibility: 
Kapil Vashisht, Carol A. Lichtensteiger, Lou A. Miller, Luis F.P. Gondim, Milton M. McAllister
Abstract: Tissue stages similar to those of Sarcocystis neurona, the causative agent of equine protozoal myeloencephalitis, were identified in skeletal muscles of a dog. The dog, a 6-year-old Labrador retriever, was seropositive for Toxoplasma gondii infection and euthanized due to a history of polymyositis and progressive muscular atrophy. Histologically, 30, variably sized, microscopic, intracellular sarcocysts were observed in 60 sections of skeletal muscles taken from the neck, fore limbs and hind limbs. The cysts were only observed in inflamed skeletal muscles, but were mostly in myocytes at the periphery of areas infiltrated with leukocytes. Ultrastructurally, the cyst wall had villar protrusions consistent with sarcocysts. Immunohistochemistry with monoclonal S. neurona antibodies demonstrated positive labeling of zoites in merozoites or schizonts in the skeletal muscle interstitium, but no labeling of the sarcocysts. Initial PCR analysis with primers amplifying a genetic sequence encoding Apicomplexan 18s rRNA, and subsequent PCR analysis with differentiating primers indicated that the genetic sequences had 100% identity with sequences reported for S. neurona.
Keywords: Sarcocystis neurona; Sarcocyst; Muscle; Dog
Rights: © 2005 Elsevier B.V. All rights reserved.
RMID: 0020101366
DOI: 10.1016/j.vetpar.2005.04.042
Appears in Collections:Animal and Veterinary Sciences publications

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