Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Advances in the use of terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes to characterize microbial communities
Author: Schutte, U.
Abo, Z.
Bent, S.
Shyu, C.
Williams, C.
Pierson, J.
Forney, L.
Citation: Applied Microbiology and Biotechnology, 2008; 80(3):365-380
Publisher: Springer-Verlag
Issue Date: 2008
ISSN: 0175-7598
Statement of
Ursel M. E. Schütte, Zaid Abdo, Stephen J. Bent, Conrad Shyu, Christopher J. Williams, Jacob D. Pierson, Larry J. Forney
Abstract: Terminal restriction fragment length polymorphism (T-RFLP) analysis is a popular high-throughput fingerprinting technique used to monitor changes in the structure and composition of microbial communities. This approach is widely used because it offers a compromise between the information gained and labor intensity. In this review, we discuss the progress made in T-RFLP analysis of 16S rRNA genes and functional genes over the last 10 years and evaluate the performance of this technique when used in conjunction with different statistical methods. Web-based tools designed to perform virtual polymerase chain reaction and restriction enzyme digests greatly facilitate the choice of primers and restriction enzymes for T-RFLP analysis. Significant improvements have also been made in the statistical analysis of T-RFLP profiles such as the introduction of objective procedures to distinguish between signal and noise, the alignment of T-RFLP peaks between profiles, and the use of multivariate statistical methods to detect changes in the structure and composition of microbial communities due to spatial and temporal variation or treatment effects. The progress made in T-RFLP analysis of 16S rRNA and genes allows researchers to make methodological and statistical choices appropriate for the hypotheses of their studies.
Keywords: T-RFLP
Microbial communities
16S rRNA genes
Multivariate statistics
Rights: © Springer-Verlag 2008
DOI: 10.1007/s00253-008-1565-4
Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.