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Type: Journal article
Title: Optimization of the Production of Biosurfactant From Iranian Indigenous Bacteria for the Reduction of Surface Tension and Enhanced Oil Recovery
Author: Amani, H.
Haghighi, M.
Sarrafzadeh, M.
Mehrnia, M.
Shahmirzaee, F.
Citation: Petroleum Science and Technology, 2011; 29(3):301-311
Publisher: Marcel Dekker Inc
Issue Date: 2011
ISSN: 1091-6466
Statement of
H. Amani, M. Haghighi, M. H. Sarrafzadeh, M. R. Mehrnia, and F. Shahmirzaee
Abstract: The optimum conditions for biosurfactant production by Iran's isolates were examined. The Taguchi method was used to identify nutritional requirements in the medium using four parameters; that is, carbon source, nitrogen, phosphorous, and salt concentrations. The use of whey, oil, and sucrose as carbon sources; NaCl as salt source; (Na2HPO4, NaH2PO 4) as phosphorous source; and (NH4)2SO 4 as nitrogen source was examined to determine bacteria optimum conditions. According to the Taguchi method using the sucrose source, the optimal conditions for Bacillus subtilis were 50 g/L NaCl, 13.53 g/L (Na 2HPO4, NaH2PO4), and 1 g/L (NH 4)2SO4; for Bacillus cereus they were 25 g/L NaCl, 13.53 g/L (Na2HPO4, NaH2PO4), and 1 g/L (NH4)2SO4; and for Pseudomonas putida they were 25 g/L NaCl, 13.53 g/L (Na2HPO4, NaH 2PO4), and 1 g/L (NH4)2SO 4. Oil displacement experiments in the micromodel at optimum conditions showed around 25% recovery of residual oil with added supernatant of Bacillus subtilis. Copyright © Taylor & Francis Group, LLC.
Keywords: Bacillus cereus
Bacillus subtilis
Pseudomonas putida
surface tension
Taguchi method
Rights: Copyright © Taylor & Francis Group, LLC
DOI: 10.1080/10916460903393963
Appears in Collections:Aurora harvest
Australian School of Petroleum publications

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