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https://hdl.handle.net/2440/66798
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Type: | Journal article |
Title: | A Comparison of mesenchymal precursor cells and amnion epithelial cells for enhancing cervical interbody fusion in an ovine model |
Author: | Goldschlager, T. Ghosh, P. Zannettino, A. Williamson, M. Rosenfeld, J. Itescu, S. Jenkin, G. |
Citation: | Neurosurgery, 2011; 68(4):1025-1034 |
Publisher: | Lippincott Williams & Wilkins |
Issue Date: | 2011 |
ISSN: | 0148-396X 1524-4040 |
Statement of Responsibility: | Tony Goldschlager, Peter Ghosh, Andrew Zannettino, Mark Williamson, Jeffrey Victor Rosenfeld, Silviu Itescu, Graham Jenkin |
Abstract: | <h4>Background</h4>Rapid, reliable fusion is the goal in anterior cervical diskectomy and fusion. Iliac crest autograft has a high rate of donor-site morbidity. Alternatives such as bone graft substitutes lack osteoinductivity, and recombinant bone morphogenetic proteins risk life-threatening complications. Both allogeneic mesenchymal precursor cells (MPCs) and amnion derived epithelial cells (AECs) have osteogenic potential.<h4>Objective</h4>To compare for the first time the capacity of MPCs and AECs to promote osteogenesis in an ovine model.<h4>Methods</h4>Five groups of 2-year-old ewes were subjected to C3-4 anterior cervical diskectomy and fusion with a Fidji interbody cage packed with iliac crest autograft alone (group A; n = 6), hydroxyapatite-tricalcium phosphate Mastergraft granules (HA/TCP) alone (group B; n = 6), HA/TCP containing 5 million MPCs (group C; n = 6), or HA/TCP containing 5 million AECs (group D; n = 5); group E was made up of age-matched nonoperative controls (n = 6). At 3 months, animals were euthanized and quantitative multislice computed tomography, functional radiography, biomechanics, histology, and histomorphometry were performed.<h4>Results</h4>No procedure- or cell-related adverse events were observed. There was significantly more fusion in the MPC group (C) than in group A, B, or D. Computed tomography scan at 3 months revealed that 5 of 6 MPC-treated animals (83%) had continuous bony bridging compared with 0 of 5 AEC-treated and only 1 of 6 autograft- and 2 of 6 HA/TCP-treated animals (P = .01).<h4>Conclusion</h4>Implantation of allogeneic MPCs in combination with HA/TCP within an interbody spacer facilitates interbody fusion after diskectomy. The earlier, more robust fusion observed with MPCs relative to autograft and HA/TCP bone substitute indicates that this approach may offer a therapeutic benefit. |
Keywords: | Amnion epithelial cell Anterior cervical diskectomy and fusion Biologics Mesenchymal stem cell Sheep Spine fusion Stem cells |
Rights: | Copyright © by the Congress of Neurological Surgeons |
DOI: | 10.1227/NEU.0b013e31820d5375 |
Published version: | http://dx.doi.org/10.1227/neu.0b013e31820d5375 |
Appears in Collections: | Aurora harvest 5 Medicine publications |
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