Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/66865
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dc.contributor.authorDo, T.-
dc.contributor.authorStephens, C.-
dc.contributor.authorTownsend, K.-
dc.contributor.authorWu, X.-
dc.contributor.authorChapman, T.-
dc.contributor.authorChin, J.-
dc.contributor.authorMcCormick, B.-
dc.contributor.authorBara, M.-
dc.contributor.authorTrott, D.-
dc.date.issued2005-
dc.identifier.citationAustralian Veterinary Journal, 2005; 83(5):293-299-
dc.identifier.issn0005-0423-
dc.identifier.issn1751-0813-
dc.identifier.urihttp://hdl.handle.net/2440/66865-
dc.description.abstract<h4>Objective</h4>To identify virulence genes in enterotoxigenic E coli (ETEC) isolates associated with diarrhoea in neonatal, 1 to 3 week-old and weaned pigs in southeast Queensland.<h4>Design</h4>Multiplex PCR and serotyping were applied to E coli isolates obtained over a 5-year period (1998-2002) from cases diagnosed at Toowoomba Veterinary Laboratory.<h4>Procedure</h4>A total of 126 isolates from 25 different Queensland piggeries were tested for haemolytic activity on 5% sheep blood agar and by multiplex PCR for the presence of five commonly recognised fimbrial (F4, F5, F6, F41 and F18) and three enterotoxin genes (STa, STb, LT). A subset of 62 representative isolates were serotyped by slide agglutination. For comparative purposes, multiplex PCR was also performed on the DNA of 31 ETEC isolates from 9 serotypes originating from piggeries in southern New South Wales.<h4>Results</h4>A total of 113 (89.7%) of the isolates from Queensland possessed ETEC virulence genes, including 14 of 15 isolates from neonatal pigs (93.3%), 18 of 23 isolates from 1 to 3 week old pigs (78.3%) and 81 of 88 isolates from weaned pigs (92.1%). F4:STa:STb:LT (serotype O149) was the most prevalent pathotype in neonatal and 1-3 week old pigs and F4:STa:STb:LT (serotype O149) and F18:STa:STb:LT (serotype O141) were most prevalent in weaned pigs. In comparison, isolates obtained from neonatal pigs from New South Wales belonged to a more diverse range of pathotypes and serotypes.<h4>Conclusion</h4>Multiplex PCR was a rapid and specific method for detecting the presence of ETEC virulence genes in porcine E coli isolates. For isolates obtained from cases of suspected colibacillosis in Queensland, growth of a heavy pure culture of haemolytic E coli was a sensitive prognostic indicator of the presence of ETEC virulence genes in the isolate. ETEC pathotypes and serotypes remained stable in Queensland piggeries over the five-year study period and appear to have changed little over the last three decades.-
dc.description.statementofresponsibilityT Do, C Stephens, K Townsend, X Wu, T Chapman, J Chin, B McCormick, M Bara and DJ Trott-
dc.language.isoen-
dc.publisherAustralian Veterinary Assn-
dc.rightsCopyright status unknown-
dc.subjectFimbriae, Bacterial-
dc.subjectAnimals-
dc.subjectAnimals, Newborn-
dc.subjectSwine-
dc.subjectEscherichia coli-
dc.subjectEscherichia coli Infections-
dc.subjectSwine Diseases-
dc.subjectDiarrhea-
dc.subjectFimbriae Proteins-
dc.subjectEscherichia coli Proteins-
dc.subjectBacterial Toxins-
dc.subjectEnterotoxins-
dc.subjectSerotyping-
dc.subjectSensitivity and Specificity-
dc.subjectPolymerase Chain Reaction-
dc.subjectVirulence-
dc.subjectGenes, Bacterial-
dc.subjectTime Factors-
dc.subjectQueensland-
dc.subjectFemale-
dc.subjectMale-
dc.titleRapid identification of virulence genes in enterotoxigenic Escherichia coli isolates associated with diarrhoea in Queensland piggeries-
dc.typeJournal article-
dc.identifier.doi10.1111/j.1751-0813.2005.tb12745.x-
pubs.publication-statusPublished-
dc.identifier.orcidTrott, D. [0000-0002-8297-5770]-
Appears in Collections:Animal and Veterinary Sciences publications
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