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Type: Journal article
Title: Antigenic differences in Newcastle Disease viruses isolated in Iran
Author: Hemmatzadeh, F.
Nayeri, B.
Tofighi, E.
Citation: International Journal of Poultry Science, 2006; 5(5):408-410
Publisher: ANSI Network
Issue Date: 2006
ISSN: 1682-8356
Statement of
F. Hemmatzadeh, B. Nayeri and E.R. Tofighi
Abstract: The samples included 30 velogenic strains of NDV, isolated from diseased chicks during 3 years period, and 2 vaccinal strains were cultured on chicken embryo and then concentrated and purified by ultra centrifugation. All samples were electrophoresed on 12.5% SDS-PAGE gels for staining and Western blotting using anti-NDV antibody. Six different antigens with molecular weigh 79, 75, 64, 40, 36 and 27 were detected in viral proteins and in some of the isolates a 48-49 kDa antigen were observed instead of a 64 kDa protein that had similarities to fusion protein in ND viruses. Western blot analysis of the antigenic patterns did not produce concise, discrete groupings, but did emphasize some relationships between virus properties and antigenicity.
Keywords: Newcastle disease virus; antigenic structure; viral proteins; western blotting; fusion protein
Rights: © Asian Network for Scientific Information 2006
RMID: 0020108334
DOI: 10.3923/ijps.2006.408.410
Appears in Collections:Animal and Veterinary Sciences publications

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