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Type: Journal article
Title: Incorporation of endothelial progenitor cells into mosaic pseudoislets
Author: Penko, D.
Mohanasundaram, D.
Sen, S.
Drogemuller, C.
Mee, C.
Bonder, C.
Coates, P.
Jessup, C.
Citation: Islets, 2011; 3(3):73-79
Publisher: Landes Bioscience
Issue Date: 2011
ISSN: 1938-2014
Statement of
Daniella Penko, Daisy Mohanasundaram, Shaundeep Sen, Christopher Drogemuller, Claire Mee, Claudine S. Bonder, P. Toby H. Coates and Claire F. Jessup
Abstract: Pancreatic islet transplantation is limited by extensive apoptosis and suboptimal function of the implanted islets in the longer term. Endothelial progenitor cells (EPC) may be ideal for enhancing both the survival and function of transplanted islets. Here, we describe for the first time the in vitro formation of rat mosaic pseudoislets comprised of pancreatic ╬▓-cells with interspersed vasculogenic EPC. Bone marrow-derived EPC displayed a similar phenotype to non-adherent EPC, recently described in the human and mouse. Mosaic pseudoislet formation was enhanced by the use of an embryoid body forming medium (BPEL) and a spin protocol. Mosaic pseudoislets maintained function in vitro and may represent an enhanced cell therapy delivery approach to enhance the survival and revascularisation of transplanted islets.
Keywords: Islets of Langerhans
Endothelial Cells
Stem Cells
Mice, Inbred NOD
Mice, SCID
Rats, Wistar
Cullin Proteins
Vascular Endothelial Growth Factor Receptor-2
Receptors, Vasopressin
Microscopy, Confocal
Islets of Langerhans Transplantation
Flow Cytometry
Cell Culture Techniques
Rights: Copyright status unknown
DOI: 10.4161/isl.3.3.15392
Grant ID:
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