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|Title:||Isolation and characterization of microsatellite markers for the Litoria ewingii complex and their use in conservation and hybridization studies|
|Citation:||Conservation Genetics Resources, 2011; 3(4):621-624|
|Katie L. Smith, Joshua M. Hale, Jeremy J. Austin, Jane Melville|
|Abstract:||Eight polymorphic microsatellite loci were identified in the Australian tree frog, Litoria ewingii, for studying patterns of hybridization with the closely related species L. paraewingi, using a microsatellite enriched genomic library. Across 55 individuals of L. ewingii and L. paraewingi, the mean number of alleles per locus was Na=9.38 and Na=7.13, respectively. All loci successfully cross-amplified in the remaining species in the Litoria ewingii complex, including the threatened sub-species L. verreauxii alpina. These loci will be useful in resolving species boundaries and exploring hybridization between species in the Litoria ewingii complex.|
|Keywords:||Hybrid zone; Microsatellite; Litoria ewingii; Litoria paraewingi; Litoria verreauxii alpina; Amphibian; South-eastern Australia; Tree frog; Species boundaries; Hybridization|
|Rights:||© Springer Science+Business Media B.V. 2011|
|Appears in Collections:||Earth and Environmental Sciences publications|
Environment Institute Leaders publications
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