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Type: Journal article
Title: Isolation and characterization of microsatellite markers for the Litoria ewingii complex and their use in conservation and hybridization studies
Author: Smith, K.
Hale, J.
Austin, J.
Melville, J.
Citation: Conservation Genetics Resources, 2011; 3(4):621-624
Publisher: Springer Netherlands
Issue Date: 2011
ISSN: 1877-7252
Statement of
Katie L. Smith, Joshua M. Hale, Jeremy J. Austin, Jane Melville
Abstract: Eight polymorphic microsatellite loci were identified in the Australian tree frog, Litoria ewingii, for studying patterns of hybridization with the closely related species L. paraewingi, using a microsatellite enriched genomic library. Across 55 individuals of L. ewingii and L. paraewingi, the mean number of alleles per locus was Na=9.38 and Na=7.13, respectively. All loci successfully cross-amplified in the remaining species in the Litoria ewingii complex, including the threatened sub-species L. verreauxii alpina. These loci will be useful in resolving species boundaries and exploring hybridization between species in the Litoria ewingii complex.
Keywords: Hybrid zone; Microsatellite; Litoria ewingii; Litoria paraewingi; Litoria verreauxii alpina; Amphibian; South-eastern Australia; Tree frog; Species boundaries; Hybridization
Rights: © Springer Science+Business Media B.V. 2011
RMID: 0020112382
DOI: 10.1007/s12686-011-9418-3
Appears in Collections:Earth and Environmental Sciences publications
Environment Institute Leaders publications

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