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Type: Journal article
Title: Immunohistochemical localisation of substance P NK1 receptor in guinea pig distal colon
Author: Harrington, A.
Hutson, J.
Southwell, B.
Citation: Neurogastroenterology and Motility, 2005; 17(5):727-737
Publisher: Blackwell Publishing Ltd
Issue Date: 2005
ISSN: 1350-1925
Statement of
A. M. Harrington, J. M. Hutson, & B. R. Southwell
Abstract: Background:  Neurokinin receptors facilitate tachykinin mediated intestinal motility and secretion. Distribution of Substance P (SP) neurokinin 1 receptor (NK1r) immunoreactivity (IR) has been previously characterized in guinea pig ileum, but not colon. This study localizes NK1rs in guinea pig distal colon. Methods:  Neurons were double labelled for NK1r and either acetylcholine transferase (ChAT), calbindin (calb), neuropeptide Y (NPY), nitric oxide synthase (NOS) or SP. The NK1r endocytosis was induced by 10−5 mol L−1 SP, septide, [SarMet] SP or neurokinin A. Results:  In guinea pig distal colon, NK1r–IR was present on 70% of submucosal neurons. Sixty-threepercent of the NK1r–IR submucosal neurons were ChAT–IR, 16% calb/SP–IR, 19% NPY–IR and 14% NOS–IR neurons. The NK1r–IR was present on 5% of myenteric neurons. Of these 63% were ChAT–IR, 16% calb–IR neurons and 25% NOS–IR. The NK1rs were also on myenteric plexus interstitial cells of Cajal and on circular muscle. Conclusion:  In guinea pig distal colon, NK1rs were on 70% of submucosal neurons including all three secretomotor neuron subtypes and sensory neurons, suggesting NK1rs have a major role in neuronal control of mucosal reflexes. The NK1rs were on few myenteric neurons but were dense on muscle cells, suggesting NK1rs affect motility through neuro-muscular rather than neuro-neuronal transmission.
Keywords: colon; enteric nervous system; guinea pig; immunohistochemistry; neurokinin 1 receptor; substance P.
Rights: © 2005 Blackwell Publishing Ltd.
RMID: 0020109035
DOI: 10.1111/j.1365-2982.2005.00680.x
Appears in Collections:Medicine publications

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