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|Title:||Immunohistochemical localisation of substance P NK1 receptor in guinea pig distal colon|
|Citation:||Neurogastroenterology and Motility, 2005; 17(5):727-737|
|Publisher:||Blackwell Publishing Ltd|
|A. M. Harrington, J. M. Hutson, & B. R. Southwell|
|Abstract:||Background: Neurokinin receptors facilitate tachykinin mediated intestinal motility and secretion. Distribution of Substance P (SP) neurokinin 1 receptor (NK1r) immunoreactivity (IR) has been previously characterized in guinea pig ileum, but not colon. This study localizes NK1rs in guinea pig distal colon. Methods: Neurons were double labelled for NK1r and either acetylcholine transferase (ChAT), calbindin (calb), neuropeptide Y (NPY), nitric oxide synthase (NOS) or SP. The NK1r endocytosis was induced by 10−5 mol L−1 SP, septide, [SarMet] SP or neurokinin A. Results: In guinea pig distal colon, NK1r–IR was present on 70% of submucosal neurons. Sixty-threepercent of the NK1r–IR submucosal neurons were ChAT–IR, 16% calb/SP–IR, 19% NPY–IR and 14% NOS–IR neurons. The NK1r–IR was present on 5% of myenteric neurons. Of these 63% were ChAT–IR, 16% calb–IR neurons and 25% NOS–IR. The NK1rs were also on myenteric plexus interstitial cells of Cajal and on circular muscle. Conclusion: In guinea pig distal colon, NK1rs were on 70% of submucosal neurons including all three secretomotor neuron subtypes and sensory neurons, suggesting NK1rs have a major role in neuronal control of mucosal reflexes. The NK1rs were on few myenteric neurons but were dense on muscle cells, suggesting NK1rs affect motility through neuro-muscular rather than neuro-neuronal transmission.|
|Keywords:||colon; enteric nervous system; guinea pig; immunohistochemistry; neurokinin 1 receptor; substance P.|
|Rights:||© 2005 Blackwell Publishing Ltd.|
|Appears in Collections:||Medicine publications|
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