Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/68427
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Type: Journal article
Title: Induced pluripotent stem cell lines derived from human gingival fibroblasts and periodontal ligament fibroblasts
Author: Wada, N.
Wang, B.
Lin, N.
Laslett, A.
Gronthos, S.
Bartold, P.
Citation: Journal of Periodontal Research, 2011; 46(4):438-447
Publisher: Blackwell Munksgaard
Issue Date: 2011
ISSN: 0022-3484
1600-0765
Statement of
Responsibility: 
Wada N, Wang B, Lin N-H, Laslett AL, Gronthos S, Bartold PM.
Abstract: Background and Objective: Human induced pluripotent stem (iPS) cells, which have similar properties to human embryonic stem (hES) cells, have been generated from neonatal and adult human dermal fibroblasts by reprogramming. iPS cells have high pluripotency and differentiation potential, and may be a potential autologous stem cell source for future regenerative therapy. Material and Methods: iPS cell lines from human gingival fibroblasts and, for the first time, from periodontal ligament fibroblasts, were generated by reprogramming using a retroviral transduction cocktail of OCT3/4, SOX2, KLF4 and c-MYC. iPS induction was investigated through expression of the embryonic stem cell markers SSEA4, OCT4, NANOG, GCTM-2, TG30 and TRA-1-60. Following in vitro differentiation, the expression of genes for differentiation markers for ectoderm (SOX1, PAX6), mesoderm [RUNX1, T(Brachyury)] and endoderm (GATA4, AFP) was assessed by real-time RT-PCR. The ability to form teratomas following implantation into mouse testes was assessed by histology. Results: Human gingival fibroblast- and periodontal ligament fibroblast-derived iPS cells showed similar characteristics to hES cells. Both sets of iPS cells displayed colony morphology comparable to that of hES cells and expressed the hES cell-associated cell-surface antigens, SSEA3, SSEA4, GCTM-2, TG30 (CD9) and Tra-1-60, and the hES cell marker genes, OCT4, NANOG and GDF3. These iPS cells showed differentiation potential to form embryoid bodies in vitro and expressed genes for endoderm, ectoderm and mesoderm. Teratoma formation following implantation into mouse testes was observed. Conclusion: These results demonstrate that iPS cells can be successfully generated from adult human gingival and periodontal ligament fibroblasts.
Keywords: periodontal ligament; gingiva; stem cell; induced pluripotent stem cell
Rights: © 2011 John Wiley & Sons A/S
RMID: 0020111251
DOI: 10.1111/j.1600-0765.2011.01358.x
Appears in Collections:Dentistry publications

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